@misc{10481/72935,
year = {2021},
month = {6},
url = {http://hdl.handle.net/10481/72935},
abstract = {Research question: The semen harbours a diverse range of microorganisms. The origin of the seminal microbes, however,
has not yet been established. Do testicular spermatozoa harbour microbes and could they potentially contribute to the
seminal microbiome composition?
Design: The study included 24 samples, comprising a total of 307 testicular maturing spermatozoa. A high-throughput
sequencing method targeting V3 and V4 regions of 16S rRNA gene was applied. A series of negative controls together with
stringent in-silico decontamination methods were analysed.
Results: Between 50 and 70% of all the detected bacterial reads accounted for contamination in the testicular sperm samples.
After stringent decontamination, Blautia (P = 0.04), Cellulosibacter (P = 0.02), Clostridium XIVa (P = 0.01), Clostridium XIVb
(P = 0.04), Clostridium XVIII (P = 0.02), Collinsella (P = 0.005), Prevotella (P = 0.04), Prolixibacter (P = 0.02), Robinsoniella
(P = 0.04), and Wandonia (P = 0.04) genera demonstrated statistically significant abundance among immature spermatozoa.
Conclusions: Our results indicate that the human testicle harbours potential bacterial signature, though in a low-biomass,
and could contribute to the seminal microbiome composition. Further, applying stringent decontamination methods is
crucial for analysing microbiome in low-biomass site.},
organization = {Copan Italia S.p.A Inc.},
organization = {Clearblue},
organization = {SPD Swiss Precision Diagnostics GmbH},
organization = {Spanish Government},
organization = {European Commission	RYC-2016-21199	
SAF2017-87526-R},
organization = {FEDER/Junta de Andalucia-Consejeria de Economia y Conocimiento: MENDO	B-CTS-500-UGR18	
Junta de Andalucia	PAIDI P20_00158},
organization = {University of Granada, Plan Propio de Investigacion 2016, Excellence actions: Units of Excellence},
organization = {Unit of Excellence on Exercise and Health (UCEES)},
organization = {Junta de Andalucia		
Consejeria de Conocimiento, Investigacion y Universidades},
organization = {European Commission	SOMM17/6107/UGR},
organization = {Spanish Government	PRE2018085440	
FPU19/01638	
FPU15/01193},
organization = {Universidad de Granada/CBUA Sequence		
BioProject	PRJNA643898},
publisher = {Elsevier},
keywords = {Male infertility},
keywords = {Microbiome},
keywords = {Microbiota},
keywords = {Semen},
keywords = {Sperm},
title = {Assessing the testicular sperm microbiome: a low-biomass site with abundant contamination},
doi = {10.1016/j.rbmo.2021.06.021},
author = {Molina Morales, Nerea and Plaza Díaz, Julio and Sola Leyva, Alberto and Mendoza Tesarik, Raquel and Galán Lázaro, Maribel and Mendoza Ladrón De Guevara, Nicolás and Tesarik, Jan and Altmae, Signe},
}