@misc{10481/72921,
year = {2019},
month = {9},
url = {http://hdl.handle.net/10481/72921},
abstract = {In this work, we have performed an in-depth study of the photophysics and solvatofluorochromism of a red-emitting Si-xanthene dye, an analog of Tokyo Magenta (TM) historically developed by Egawa et al. (Chem. Commun. 2011, 47, 4162–4164). The results show a strong dependency of the emission properties of 2-Me-4-OMe-TM on the polarity of the solvent. For instance, the dye exhibited an increase in its fluorescence lifetime with the decrease in solvent polarity. Therefore, in this work, this spectral behavior has been used as a new approach for determining the intracellular microenvironment polarity through the measurement of its fluorescence lifetime by Fluorescence Lifetime Imaging Microscopy (FLIM). Our experiments confirmed the ability of the dye to detect changes in polarity between different intracellular compartments.},
organization = {This work was funded by grants CTQ2017-85658-R, CTQ2014-55474-C2-2-R (Spanish Ministry of Economy and Competitiveness; Agencia Estatal de Investigacion, AEI; and European Regional Development Fund, ERDF), QM2012-790 (Junta de Andalucía), and a grant from the Fundación Botín.},
keywords = {Hetero-xanthene dyes},
keywords = {Solvatofluorochromism},
keywords = {Fluorescent dyes},
keywords = {Biosensors},
keywords = {Photophysics},
title = {A solvatofluorochromic silicon-substituted xanthene dye useful in bioimaging},
doi = {10.1016/j.dyepig.2019.04.024},
author = {Espinar Barranco, Laura and Luque Navarro, Pilar María and Strnad, Maja Ana and Herrero-Foncubierta, Pilar and Crovetto González, Luis and Miguel Álvarez, Delia and Girón González, María Dolores and Orte Gutiérrez, Ángel and Cuerva Carvajal, Juan Manuel and Salto González, Rafael and Alvarez-Pez, José María and Paredes Martínez, José Manuel},
}