@misc{10481/72792,
year = {2021},
month = {6},
url = {http://hdl.handle.net/10481/72792},
abstract = {A new chloride-sensitive red fluorescent protein
derived from Entacmaea quadricolor is described. We found that
mBeRFP exhibited moderate sensitivity to chloride and, via sitedirected
mutagenesis (S94V and R205Y), we increased the
chloride affinity by more than an order of magnitude (kd = 106
± 6 mM) at physiological pH. In addition, cis−trans isomerization
of the chromophore produces a dual emission band with different
chloride sensitivities, which allowed us to develop a ratiometric
methodology to measure intracellular chloride concentrations.},
organization = {MICIU/AEI/ERDF	CTQ2017-85685-R	
CTQ2017-85454-C2-1-P	
CTQ2017-86125-P},
organization = {UEQ},
organization = {Spanish Government	FPU17/04749},
organization = {Ministry of Health, Italy},
organization = {Italian Cystic Fibrosis Research Foundation	3/2019},
organization = {Fondazione Cassa Rurale Trento Rovereto	2018.256},
publisher = {American Chemical Society},
keywords = {Red fluorescent proteins},
keywords = {Chloride sensors},
keywords = {Imaging},
keywords = {Two-photon excitation microscopy},
keywords = {Genetically encoded sensors},
keywords = {Intracellular sensors},
title = {New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses},
doi = {10.1021/acssensors.1c00094},
author = {Salto González, Rafael and Girón González, María Dolores and Puente Muñoz, Virginia and Vílchez Rienda, José Dámaso and Espinar Barranco, Laura and Valverde Pozo, Javier and Paredes Martínez, José Manuel},
}