@misc{10481/72669,
year = {2022},
month = {1},
url = {http://hdl.handle.net/10481/72669},
abstract = {The small molecule 8-methoxy-2-oxo-1,2,4,5-
tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves
as a reactive non-fluorescent Michael acceptor, which after reaction
with thiols becomes fluorescent, and an efficient Eu3+ antenna,
after self-assembling with this cation in water. This behavior makes
2b a highly selective GSH biosensor, which has demonstrated high
potential for studies in murine and human cells of the immune
system (CD4+ T, CD8+ T, and B cells) using flow cytometry. GSH
can be monitored by the fluorescence of the product of addition to
2b (445 nm) or by the luminescence of Eu3+ (592 nm). 2b was
able to capture baseline differences in GSH intracellular levels
among murine and human CD4+ T, CD8+ T, and B cells. We also
successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of
CD4+ naiv̈ e T cells into regulatory T cells (TREG).},
organization = {MCIN/AEI/FEDER "Una manera de hacer Europa"	CTQ2017-85658-R
BFU2015-67284-R
PID2019-104366RB-C22},
organization = {MCIN/AEI	PID2020-114256RB-I00},
organization = {FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades	A-FQM-386-UGR20},
organization = {CSIC grant	201580E073},
organization = {Universidad de Granada/CBUA},
publisher = {American Chemical Society},
keywords = {Glutathione},
keywords = {Luminescent sensor},
keywords = {Self-assembled antenna},
keywords = {Lanthanide},
keywords = {Time-resolved luminescence},
keywords = {Flow cytometry},
keywords = {T cells},
keywords = {TREG},
title = {Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells},
doi = {10.1021/acssensors.1c02439},
author = {Fueyo González, Francisco and Espinar Barranco, Laura and Crovetto González, Luis and Paredes Martínez, José Manuel and Orte Gutiérrez, Ángel and González Vera, Juan Antonio},
}