@misc{10481/72590, year = {2021}, month = {12}, url = {http://hdl.handle.net/10481/72590}, abstract = {In a recent work, we reported a methodology for imaging the different stages of amyloid aggregation in quantitative multiparametric dual-color fluorescence lifetime imaging (FLIM) and superresolution microscopy by using a pair of dyes capable of binding aggregates and undergoing subsequent intra-aggregate energy transfer (FRET) (RuizArias et al. Sensors Actuat. B, 2022, 350:130882). In this microarticle we present the optimization process for choosing the best pair of dyes through a screening of different naphthalimides and quinolimides and other known amyloid-binding dyes.}, organization = {Spanish Ministerio de Educación y Formación}, organization = {Grant CTQ2017–85658-R funded by MCIN/AEI/10.13039/501100011033/FEDER “Una manera de hacer Europa”}, organization = {Grants PID2019–104366RB-C22 and PID2020–114256RBI00 funded by MCIN/AEI/10.13039/ 501100011033}, publisher = {Elsevier}, keywords = {Amyloid fibrils}, keywords = {Protein aggregation}, keywords = {Solvatochromic dyes}, keywords = {Biomarkers}, keywords = {FLIM}, keywords = {STED microscopy}, title = {Selecting FRET pairs for visualizing amyloid aggregation}, doi = {10.1016/j.rechem.2021.100275}, author = {Ruiz Arias, Álvaro and Jurado Palomares, Rocío and Fueyo Gonzalez, Francisco and Herranz, Rosario and Gálvez Rodríguez, Natividad and González Vera, Juan Antonio and Orte Gutiérrez, Ángel}, }