@misc{10481/71579,
year = {2021},
month = {9},
url = {http://hdl.handle.net/10481/71579},
abstract = {Unraveling cellular physiological processes via luminescent probes that target specific cellular microenvironments is quite challenging due to the uneven distribution of probes. Herein, we designed a new dynamic excimer (DYNEX) imaging method that involves the sensitive detection of nanosecond-scale dynamic molecular contacts of a fluorescent acridone derivative and reveals the cell microenvironment polarity. Using our method, we specifically tracked cell lipid droplets in fibroblast colon carcinoma cells. These organelles play a central role in metabolic pathways, acting as energy reservoirs in regulatory processes. DYNEX imaging provides the inner polarity of cell lipid droplets, which can be related to lipid contents and metabolic dysfunctions. This new methodology will inspire development of novel multidimensional fluorescent sensors that are able to provide target-specific and orthogonal information at the nanosecond scale.},
organization = {CBUA},
organization = {MICIU},
organization = {Universidad de Granada},
organization = {Universidad de Jaén},
organization = {Agencia Estatal de Investigación},
organization = {European Regional Development Fund FQM-337},
organization = {Junta de Andalucía PIUJA 2019-20},
publisher = {American Chemical Society},
keywords = {Cell microenvironment},
keywords = {Excimer imaging},
keywords = {Fluorescent probes},
keywords = {Imaging agents},
keywords = {Lipids},
keywords = {Multiparametric microscopy},
title = {Dynamic Excimer (DYNEX) Imaging of Lipid Droplets},
doi = {10.1021/acssensors.1c01206},
author = {González García, María del Carmen and Salto Girón, Carmen and Herrero-Foncubierta, Pilar and Peña-Ruiz, Tomás and Girón González, María Dolores and Salto González, Rafael and Pérez Lara, Francisco Ángel and Navarro, A and García-Fernández, Emilio and Orte Gutiérrez, Ángel},
}