@misc{10481/71523,
year = {2021},
url = {http://hdl.handle.net/10481/71523},
abstract = {Critical defects of the mandibular bone are very difficult to manage with currently available
materials and technology. In the present work, we generated acellular and cellular substitutes
for human bone by tissue engineering using nanostructured fibrin–agarose biomaterials, with and
without adipose-tissue-derived mesenchymal stem cells differentiated to the osteogenic lineage using
inductive media. Then, these substitutes were evaluated in an immunodeficient animal model of
severely critical mandibular bone damage in order to assess the potential of the bioartificial tissues to
enable bone regeneration. The results showed that the use of a cellular bone substitute was associated
with a morpho-functional improvement of maxillofacial structures as compared to negative controls.
Analysis of the defect site showed that none of the study groups fully succeeded in generating
dense bone tissue at the regeneration area. However, the use of a cellular substitute was able to
improve the density of the regenerated tissue (as determined via CT radiodensity) and form isolated
islands of bone and cartilage. Histologically, the regenerated bone islands were comparable to control
bone for alizarin red and versican staining, and superior to control bone for toluidine blue and
osteocalcin in animals grafted with the cellular substitute. Although these results are preliminary,
cellular fibrin–agarose bone substitutes show preliminary signs of usefulness in this animal model of
severely critical mandibular bone defect.},
organization = {Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i)},
organization = {Instituto de Salud Carlos III},
organization = {Ministerio de Ciencia e Innovación},
organization = {European Regional Development Fund (ERDF-FEDER)},
organization = {Consejería de Salud y Familias,
Junta de Andalucía, Spain},
publisher = {MDPI},
keywords = {Bone},
keywords = {Tissue engineering},
keywords = {Fibrin-agarose},
keywords = {Mandible},
keywords = {Regeneration},
title = {Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect},
doi = {10.3390/polym13223939},
author = {Martín Piedra, Miguel Ángel and Gironés Camarasa, Belén and España López, Antonio and Fernández-Valadés Gámez, Ricardo and Blanco Elices, Cristina and Garzón Bello, Ingrid Johanna and Alaminos Mingorance, Miguel and Fernández Valadés, Ricardo},
}