@misc{10481/71349,
year = {2021},
url = {http://hdl.handle.net/10481/71349},
abstract = {Patients with severe limbal damage and limbal stem cell deficiency are a therapeutic challenge. We evaluated four decellularization protocols applied to the full-thickness and half-thickness
porcine limbus, and we used two cell types to recellularize the decellularized limbi. The results
demonstrated that all protocols achieved efficient decellularization. However, the method that best
preserved the transparency and composition of the limbus extracellular matrix was the use of 0.1%
SDS applied to the half-thickness limbus. Recellularization with the limbal epithelial cell line SIRC
and human adipose-derived mesenchymal stem cells (hADSCs) was able to generate a stratified
epithelium able to express the limbal markers p63, pancytokeratin, and crystallin Z from day 7 in
the case of SIRC and after 14–21 days of induction when hADSCs were used. Laminin and collagen
IV expression was detected at the basal lamina of both cell types at days 14 and 21 of follow-up.
Compared with control native limbi, tissues recellularized with SIRC showed adequate picrosirius
red and alcian blue staining intensity, whereas limbi containing hADSCs showed normal collagen
staining intensity. These preliminary results suggested that the limbal substitutes generated in this
work share important similarities with the native limbus and could be potentially useful in the future.},
organization = {Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i) of the Spanish Ministry of Economy and Competitiveness
(Instituto de Salud Carlos III), Grants FIS PI20/0317 and ICI21-00010, cofinanced by FEDER funds
(European Union). This work was also supported by grant PI-0086-2020 from Consejería de Salud y
Familias, Junta de Andalucía, Spain, and grant B-CTS-504-UGR20 (Proyectos de I+D+i en el marco
del Programa Operativo FEDER Andalucía 2014–2020) from the University of Granada, Consejería
de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía, and
European Union (cofinanced by FEDER funds).},
publisher = {MDPI},
keywords = {Corneal limbus},
keywords = {Decellularized xenograft},
keywords = {Recellularization},
keywords = {Mesenchymal stem cells},
title = {Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds},
doi = {10.3390/pharmaceutics13101718},
author = {Sánchez Porras, David and Caro Magdaleno, Manuel and González Gallardo, Carmen and García García, Óscar Darío and Garzón Bello, Ingrid Johanna and Carriel Araya, Víctor and Campos Sánchez, Fernando and Alaminos Mingorance, Miguel},
}