@misc{10481/71246,
year = {2021},
month = {9},
url = {http://hdl.handle.net/10481/71246},
abstract = {Moringa oleifera, Tropaeolum tuberosum and Annona cherimola are medicinal plants traditionally used in Ecuador.
However, their therapeutic properties are not completely known. We analyzed chromatographically ethanolic
extracts of the seeds of M. oleifera, A. cherimola and the tubers of T. tuberosum; all presented a high content of
polyphenols. The extract of A. cherimola showed the highest antioxidant activity and M. oleifera had the highest
capacity to enhance the activity of detoxifying enzymes such as glutathione S-transferase and quinone oxidoreductase.
The antitumor effect of these extracts was evaluated in vitro with colorectal cancer (CRC) cell lines
T84, HCT-15, SW480 and HT-29, as well as with cancer stem cells (CSCs). A. cherimola and M. oleifera extracts
presented the lowest IC50 in T-84 and HCT-15 (resistant) cells, respectively, as well as the highest level of inhibition
of proliferation in multicellular tumor spheroids of HCT-15 cells. The inhibitory effect on CSCs is
noteworthy because in vivo, these cells are often responsible for cancer recurrences and resistance to chemotherapy.
Moreover, all extracts showed a synergistic activity with 5-Fu. The antiproliferative mechanism of the
extracts was related to overexpression of caspases 9, 8 and 3 and increased production of reactive oxygen species.
In addition, we observed cell death by autophagy in M. oleifera and T. tuberosum extracts. Therefore, these
ethanolic extracts are excellent candidates for future molecular analysis of the presence of bioactive compounds
and in vivo studies which could improve colon cancer therapy.},
organization = {Granada Universit	CTS-107	
AGR145},
organization = {2018 Research Initiation Grants Program for Master Students of the Vice-Rectorate for Research and Knowledge Transfer of the University of Granada},
publisher = {Elsevier},
keywords = {Moringa oleifera},
keywords = {Tropaeolum tuberosum},
keywords = {Annona cherimola},
keywords = {Ethanolic extract},
keywords = {Colon cancer},
keywords = {5-fluorouracil},
title = {Antioxidant and antiproliferative potential of ethanolic extracts from Moringa oleifera, Tropaeolum tuberosum and Annona cherimola in colorrectal cancer cells},
doi = {10.1016/j.biopha.2021.112248},
author = {Fuel, Marco and Mesas Hernández, Cristina and Martínez Martínez, Rosario and Ortiz Quesada, Raúl and Quiñonero Muñoz, Francisco José and Prados Salazar, José Carlos and Porres Foulquie, Jesús María and Melguizo Alonso, Consolación},
}