@misc{10481/71004,
year = {2021},
url = {http://hdl.handle.net/10481/71004},
abstract = {Background: A lifelong strict gluten-free diet is the only available treatment for celiac disease, but total exclusion of gluten is difficult to achieve. The aim of this study was to determine the
range of time and the amount of gluten immunogenic peptides (GIP) excreted in urine after specific
gluten ingestions. Methods: 20 healthy participants followed the same diet for 12 days in which 50
mg and 2 g of gluten were ingested and all the urinations were collected. GIP were analyzed by
lateral flow immunoassay (LFIA) tests and quantified using an LFIA reader. Results: GIP were detected in 15% and 95% of participants after 50 mg and 2 g gluten intakes, respectively. The higher
frequency and concentration of GIP was found between 6 and 9 h after both gluten ingestions. The
ranges of detection were 3–12 h (50 mg) and 0–15 h (2 g). Conclusions: An increase in the frequency
of urine tests may be a suitable approach to avoid false negative results. The use of the LFIA test in
three urine samples collected at different times may show a sensitivity of 19.6% for a gluten ingestion like 50 mg, increasing to 93% after 2 g consumption.},
organization = {Ministerio de Ciencia e Innovación (DI-16-08943)
and Junta de Andalucía, Consejería de Economía, Conocimiento, Empresas y Universidad},
organization = {FEDER funds (AT17_5489_USE)},
publisher = {MDPI},
keywords = {Gluten immunogenic peptides},
keywords = {Gluten excretion urine},
keywords = {Gluten-free diet monitoring},
keywords = {Celiac disease},
title = {Dynamics and Considerations in the Determination of the Excretion of Gluten Immunogenic Peptides in Urine: Individual Variability at Low Gluten Intake},
doi = {10.3390/nu13082624},
author = {Coto, Laura and Sousa, Carolina and Cebolla, Angel},
}