@misc{10481/70659,
year = {2021},
month = {7},
url = {http://hdl.handle.net/10481/70659},
abstract = {Expression of Wilms’ tumor suppressor transcription factor (WT1) in the embryonic
epicardium is essential for cardiac development, but its myocardial expression is
little known. We have found that WT1 is expressed at low levels in 20–25% of the
embryonic cardiomyocytes. Conditional ablation of WT1 using a cardiac troponin T
driver (Tnnt2Cre) caused abnormal sinus venosus and atrium development, lack of
pectinate muscles, thin ventricular myocardium and, in some cases, interventricular
septum and cardiac wall defects, ventricular diverticula and aneurisms. Coronary
development was normal and there was not embryonic lethality, although survival of
adult mutant mice was reduced probably due to perinatal mortality. Adult mutant mice
showed electrocardiographic anomalies, including increased RR and QRS intervals, and
decreased PR intervals. RNASeq analysis identified differential expression of 137 genes
in the E13.5 mutant heart as compared to controls. GO functional enrichment analysis
suggested that both calcium ion regulation and modulation of potassium channels
are deeply altered in the mutant myocardium. In summary, together with its essential
function in the embryonic epicardium, myocardial WT1 expression is also required for
normal cardiac development.},
organization = {Spanish Ministry of Economy, Industry and Competitivity	BFU2017-83907-P	
PID2019-107492GB-I00},
organization = {Junta de Andalucia	PC0066?2017/PC-0081-2017},
organization = {Instituto de Salud Carlos III-TERCEL network	RD16/0011/0030	
Instituto de Salud Carlos III-CIBERCV "Enfermedades Cardiovasculares"	CB16/11/00360},
organization = {Junta de Andalucia	UMA18-FEDERJA-146},
publisher = {Frontiers Research Foundation},
keywords = {Wilms' tumor supressor gene},
keywords = {Cardiomyocytes},
keywords = {Cardiac development},
keywords = {Calcium homeostasis},
keywords = {Potassium channels},
title = {Deletion of the Wilms’ Tumor Suppressor Gene in the Cardiac Troponin-T Lineage Reveals Novel Functions of WT1 in Heart Development},
doi = {10.3389/fcell.2021.683861},
author = {Díaz del Moral, Sandra and Hernández Torres, Francisco and Aránega, Amelia Eva},
}