@misc{10481/70531,
year = {2021},
month = {7},
url = {http://hdl.handle.net/10481/70531},
abstract = {Hawthorn leaves are a rich source of phenolic compounds that possess beneficial activities
for human health. Ultrasonic-assisted extraction (UAE) is an extraction technique frequently used
for the isolation of phenolic compounds in plants. Thus, in this study, a Box–Behnken design
was used to optimize UAE conditions such as the percentage of acetone, the extraction time and
solvent-to-solid ratio (v/w) in order to obtain the maximum content of total compounds by Folin–
Ciocalteu and the maximum in vitro antioxidant activity by DPPH, ABTS and FRAP assays in
Crataegus monogyna leaves. The optimum conditions to obtain the highest total phenolic content
and antioxidant activities were 50% acetone, 55 min and 1/1000 (w/v). A total of 30 phenolic
compounds were identified and quantified in C. monogyna leaf extract obtained at these optimum
UAE conditions. HPLC-MS allows the identification and quantification of 19 phenolic compounds
and NP-HPLC-FLD analyses showed the presence of 11 proanthocyanidins. According to the results,
the most concentrated phenolic compounds in C. monogyna leaf extract obtained at optimum UAE
conditions were phenolic acid derivatives such as protocatechuic acid-glucoside, dihydroxy benzoic
acid pentoside and chlorogenic acid, flavones such as 2”-O-rhamnosyl-C-hexosyl-apigenin, flavonols
such as hyperoside and isoquercetin and proanthocyanidins such as monomer and dimer. As a result,
the optimized UAE conditions could be used to obtain an extract of C. monogyna leaves enriched with
phenolic compounds.},
organization = {Spanish Ministry of Economy and Competitiveness (MINECO) for the "Ramon y Cajal" contract	RYC-2015-18795},
publisher = {MDPI},
keywords = {Ultrasonic-assisted extraction},
keywords = {Hawthorn (Crataegus monogyna)},
keywords = {HPLC-ESI-TOF-MS},
keywords = {HPLC-FLD},
keywords = {Box-Behnken Design},
title = {Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus monogyna Leaves},
doi = {10.3390/molecules26154536},
author = {Martín García, Beatriz and Razola Díaz, María del Carmen and Gómez Caravaca, Ana María and Benítez Cruz, Guillermo and Verardo, Vito},
}