@misc{10481/70053,
year = {2021},
month = {7},
url = {http://hdl.handle.net/10481/70053},
abstract = {Histidine metabolism is a key pathway physiologically involved in satiety, recognition
memory, skin, and neural protection and allergic diseases. Microbiologicallyproduced
imidazole propionate induces type II diabetes and interferes with glucose
lowering drugs. Despite their determinant health implications, no single
method simultaneously assesses histidine metabolites in urine, feces, and microbiota.
The aim of this study was to develop a simple, rapid, and sensitive method
for the determination of histidine and its major bioactive metabolites histamine,
N-acetylhistamine, imidazole-4-acetate, cis-urocanate, trans-urocanate, glutamate
and imidazole propionate, using ultrahigh-performance liquid chromatography
with electrospray ionization tandem mass spectrometry. An innovative simple
extraction method from small aliquots of human and mice urine, feces and microbial cell extracts was coupled to separation in a 6.5 min chromatographic
run. The successful performance allowed accurate and precise quantification of all
metabolites in mouse feces, suggesting broad exchange of histidine metabolites
between the gut and mice. Higher urine histamine, histamine to histidine ratio,
and imidazole-4-acetate pointed to an underlying inflammatory or allergic process
in mice compared to human subjects. N-acetylhistamine and imidazole propionate
were detected in human and mouse feces, confirming its origin from gut microbial
metabolism. Our novel and robust analytical method captured histidine metabolism
in a single assay that will facilitate broad and deep histidine metabolic
phenotyping assessing the impact of microbiota on host health in large-scale
human observational and interventional studies.},
organization = {European Food Safety Authority},
organization = {FEDER-Infraestructure Consejeria de Economia, Conocimiento, Empresas y Universidad	IE_2019-198},
publisher = {Wiley-Blackwell Publishing},
keywords = {Feces},
keywords = {Histidine pathway},
keywords = {Microbiota},
keywords = {UHPLC-ESI-MS/MS},
keywords = {Urine},
title = {Rapid and simultaneous determination of histidine metabolism intermediates in human and mouse microbiota and biomatrices},
doi = {10.1002/biof.1766},
author = {Acuña Morales, Inmaculada and Cantarero Malagón, Antonio Samuel and López Moreno, Ana and Aguilera Gómez, Margarita and Campoy Folgoso, Cristina and Suárez García, Antonio Francisco},
}