@misc{10481/69363,
year = {2021},
month = {5},
url = {http://hdl.handle.net/10481/69363},
abstract = {Type 2 diabetes (T2D) is a rising global health problem mainly caused by obesity and a sedentary lifestyle. In healthy individuals, white adipose tissue (WAT) has a relevant homeostatic role in glucose metabolism, energy storage, and endocrine signaling. Mast cells contribute to these functions promoting WAT angiogenesis and adipogenesis. In patients with T2D, inflammation dramatically impacts WAT functioning, which results in the recruitment of several leukocytes, including monocytes, that enhance this inflammation. Accordingly, the macrophages population rises as the WAT inflammation increases during the T2D status worsening. Since mast cell progenitors cannot arrive at WAT, the amount of WAT mast cells depends on how the new microenvironment affects progenitor and differentiated mast cells. Here, we employed a flow cytometry-based approach to analyze the number of mast cells from omental white adipose tissue (o-WAT) and subcutaneous white adipose tissue (s-WAT) in a cohort of 100 patients with obesity. Additionally, we measured the number of mast cell progenitors in a subcohort of 15 patients. The cohort was divided in three groups: non-T2D, pre-T2D, and T2D. Importantly, patients with T2D have a mild condition (HbA1c <7%). The number of mast cells and mast cell progenitors was lower in patients with T2D in both o-WAT and s-WAT in comparison to subjects from the pre-T2D and non-T2D groups. In the case of mast cells in o-WAT, there were statistically significant differences between non-T2D and T2D groups (p = 0.0031), together with pre-T2D and T2D groups (p=0.0097). However, in s-WAT, the differences are only between non-T2D and T2D groups (p=0.047). These differences have been obtained with patients with a mild T2D condition. Therefore, little changes in T2D status have a huge impact on the number of mast cells in WAT, especially in o-WAT. Due to the importance of mast cells in WAT physiology, their decrease can reduce the capacity of WAT, especially o-WAT, to store lipids and cause hypoxic cell deaths that will trigger inflammation.},
organization = {Instituto de Salud Carlos III

PI15/01361},
organization = {MINECO, Madrid 

DPI2017-84439-R},
organization = {European Commission},
organization = {Spanish Ministry of Science and Innovation (" Formacion de profesorado universitario" grant) 

FPU18/04432
B16.56.1},
organization = {University of Granada ("Becas de iniciacion a la investigacion del plan propio de la UGR")},
publisher = {Frontiers Research Foundation},
keywords = {Mast cell},
keywords = {T2D},
keywords = {Adipose tissue},
keywords = {Obesity},
keywords = {Flow cytometry},
keywords = {Angiogenesis},
keywords = {Inflammation},
keywords = {Adipogenesis},
title = {In Patients With Obesity, the Number of Adipose Tissue Mast Cells Is Significantly Lower in Subjects With Type 2 Diabetes},
doi = {10.3389/fimmu.2021.664576},
author = {López Pérez, David and Redruello Romero, Anais and García Rubio, Jesús and Arana, Carlos and García Escudero, Luis A. and Tamayo, Francisco and Puentes Pardo, José David and Moreno San Juan, Sara and Salmerón Escobar, Francisco Javier and Blanco Morón, Armando and Gálvez Peralta, Julio Juan and León López, Josefa and Carazo Gallego, Ángel},
}