@misc{10481/68867,
year = {2021},
url = {http://hdl.handle.net/10481/68867},
abstract = {One of the most challenging environmental threats of the last two decades is the effects of
emerging pollutants (EPs) such as pharmaceutical compounds or industrial additives. Diclofenac
and bisphenol A have regularly been found in wastewater treatment plants, and in soils and water
bodies because of their extensive usage and their recalcitrant nature. Due to the fact of this adversity,
fungal communities play an important role in being able to safely degrade EPs. In this work, we
obtained a sewage sludge sample to study both the culturable and non-culturable microorganisms
through DNA extraction and massive sequencing using Illumina MiSeq techniques, with the goal
of finding degraders adapted to polluted environments. Afterward, degradation experiments on
diclofenac and bisphenol A were performed with the best fungal degraders. The analysis of bacterial
diversity showed that Dethiosulfovibrionaceae, Comamonadaceae, and Isosphaeraceae were the
most abundant families. A predominance of Ascomycota fungi in the culturable and non-culturable
population was also detected. Species such as Talaromyces gossypii, Syncephalastrum monosporum,
Aspergillus tabacinus, and Talaromyces verruculosus had remarkable degradation rates, up to 80% of
diclofenac and bisphenol A was fully degraded. These results highlight the importance of characterizing autochthonous microorganisms and the possibility of selecting native fungal microorganisms
to develop tailored biotransformation technologies for EPs.},
organization = {Ministry of Economy and Competitiveness (MINECO)},
organization = {European Regional Development Fund (ERDF) funds, grant number[(CTM2017-84332-R (MINECO/
AEI/FEDER/UE))},
organization = {Consejo Nacional
de Ciencia y Tecnología (CONACyT, México), grant number (CVU 230592- I1200/94/2020 and 377965,
respectively)},
organization = {GAP was funded by Consejo Nacional de Ciencia y
Tecnología (CONACyT, Mexico) grant number (CVU 772485-739637)},
publisher = {MDPI},
keywords = {Sewage sludge},
keywords = {Pharmaceutical active compounds},
keywords = {Bioremediation},
keywords = {Endocrine disruptors},
keywords = {Shotgun-sequencing technologies},
keywords = {Fungi},
keywords = {Bacteria},
title = {Evaluation of the Potential of Sewage Sludge Mycobiome to Degrade High Diclofenac and Bisphenol-A Concentrations},
doi = {10.3390/toxics9060115},
author = {Conejo Saucedo, Ulises and Ledezma Villanueva, Alejandro and Ángeles de Paz, Gabriela and Herrero Cervera, Mario and Calvo Sáinz, Concepción and Aranda Ballesteros, Elisabet},
}