@misc{10481/68604,
year = {2021},
url = {http://hdl.handle.net/10481/68604},
abstract = {High saturated fat diets have been associated with the development of obesity and hypertension, along with other pathologies related to the metabolic syndrome. In contrast, the Mediterranean
diet, characterized by its high content of monounsaturated fatty acids, has been proposed as a
dietary factor capable of positively regulating cardiovascular function. These effects have been linked
to changes in the local renal renin angiotensin system (RAS) and the activity of the sympathetic
nervous system. The main goal of this study was to analyze the role of two dietary fat sources on
aminopeptidases activities involved in local kidney RAS. Male Wistar rats (six months old) were fed
during 24 weeks with three different diets: the standard diet (S), the standard diet supplemented with
virgin olive oil (20%) (VOO), or the standard diet enriched with butter (20%) plus cholesterol (0.1%)
(Bch). Kidney samples were separated in medulla and cortex for aminopeptidase activities (AP) assay.
Urine samples were collected for routine analysis by chemical tests. Aminopeptidase activities were
determined by fluorometric methods in soluble (sol) and membrane-bound (mb) fractions of renal
tissue, using arylamide derivatives as substrates. After the experimental period, the systolic blood
pressure (SBP) values were similar in standard and VOO animals, and significantly lower than in
the Bch group. At the same time, a significant increase in GluAP and IRAP activities were found
in renal medulla of Bch animals. However, in VOO group the increase of GluAP activity in renal
medulla was lower, while AspAP activity decreased in the renal cortex. Furthermore, the VOO diet
also affected other aminopeptidase activities, such as TyrAP and pGluAP, related to the regulation of
the sympathetic nervous system and the metabolic rate. These results support the beneficial effect of
VOO in the regulation of SBP through changes in local AP activities of the kidney.},
organization = {ACCIÓN 1 PAIUJA 2019 2020: BIO221},
publisher = {MDPI},
keywords = {Renal–aminopeptidase activities},
keywords = {Systolic blood pressure},
keywords = {High-fat diet},
keywords = {Virgin olive oil},
title = {Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats},
doi = {10.3390/ ijms22105388},
author = {Domínguez Vías, German},
}