@misc{10481/68585, year = {2020}, month = {7}, url = {http://hdl.handle.net/10481/68585}, abstract = {Recent studies have shown the potential of magnetic hyperthermia in cancer treatments. However, the underlying mechanisms involved have not been yet fully described. In particular, the cell death related to magnetic hyperthermia observed in cultures incubated with low concentration of magnetic nanoparticles and under a low intensity alternating magnetic field, in which a macroscopic temperature rise is not observed, is still not understood. In the present study, we investigate the production of intracellular Reactive Oxygen Species (ROS) as a mechanism to induce cell death under these conditions. In this study, the production and influence of ROS on the viability of HepG2 human hepatoma cells (used as a model cell line) are analyzed under the application of variable magnetic fields using hyperthermia agents, such as biomimetic magnetic nanoparticles (BMNPs) mediated by magnetosome MamC protein fromMagnetococcus marinusMC-1. The results show that intracellular ROS production increases up to similar to 90% following upon the exposure of AMF to HepG2 cells containing BMNPs, which could determine the loss of cell viability (up to similar to 40% reduction) without a significant rise in temperature. Such ROS production is linked to mitochondrial dysfunction caused by the application of AMF to cells containing BMNPs.}, organization = {Spanish Government CGL2016-76723}, organization = {Spanish Government RYC-2014-16901}, organization = {Junta de Andalucia}, organization = {Programa Operativo FEDER 2014-2020 A1-FQM-341-UGR18 C-FQM-497-UGR18 A-BIO-376-UGR18}, organization = {Andalusian regional government CTS-236}, organization = {Ministerio de Ciencia, Innovacion y Universidades (Spain) PRE2018-085440}, organization = {Ministerio de Educacion, Ciencia y Deporte y Competitividad (Spain) FPU16_04580}, organization = {Unidad Cientifica de Excelencia of the University of Granada UCE-PP2016-05}, publisher = {Royal Society of Chemistry}, title = {Reactive oxygen species (ROS) production in HepG2 cancer cell line through the application of localized alternating magnetic field}, doi = {10.1039/d0tb01306d}, author = {Sola Leyva, Alberto and Jabalera Ruz, Ylenia María and Chico Lozano, María Ángeles and Carrasco Jiménez, María Paz and Iglesias Salto, Guillermo Ramón and Jiménez López, Concepción}, }