@misc{10481/68520,
year = {2021},
url = {http://hdl.handle.net/10481/68520},
abstract = {Several models of bioartificial human urothelial mucosa (UM) have been described recently.
In this study, we generated novel tubularized UM substitutes using alternative sources of cells.
Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter
were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC)
were used to generate an epithelial-like layer on top. Three differentiation media were used for 7
and 14 days. Results showed that the biofabrication methods used here succeeded in generating
a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top,
especially using a medium containing epithelial growth and differentiation factors (EM), although
differentiation was not complete. At the functional level, UM substitutes were able to synthesize
collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not
reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days
of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tightjunction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm
the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods
explored here were able to generate a potential substitute of the human UM for future clinical use.},
organization = {CTS-115 Tissue Engineering Group and by the Spanish
Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministry of Science
and Innovation, Instituto de Salud Carlos III, grant FIS PI21/0981 (cofinanced by FEDER funds,
European Union).},
publisher = {MDPI},
keywords = {Urothelial mucosa},
keywords = {Human Wharton jelly mesenchymal stromal cells},
keywords = {Biofabrication},
title = {Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells},
doi = {10.3390/polym13101568},
author = {Garzón Bello, Ingrid Johanna and Jaimes Parra, Boris Damián and Cózar Olmo, José Manuel and Sánchez Quevedo, María Del Carmen and Sánchez-Montesinos García, Indalecio and Fernández Valadés, Ricardo and Campos Sánchez, Fernando and Alaminos Mingorance, Miguel},
}