@misc{10481/68432,
year = {2021},
url = {http://hdl.handle.net/10481/68432},
abstract = {Human gut microbiota harbors numerous microbial species with molecular enzymatic
potential that impact on the eubiosis/dysbiosis and health/disease balances. Microbiota species
isolation and description of their specific molecular features remain largely unexplored. In the
present study, we focused on the cultivation and selection of species able to tolerate or biodegrade
the endocrine disruptor bisphenol A (BPA), a xenobiotic extensively found in food plastic containers.
Chemical xenobiotic addition methods for the directed isolation, culturing, Whole Genome Sequencing (WGS), phylogenomic identification, and specific gene-encoding searches have been applied to
isolate microorganisms, assess their BPA metabolization potential, and describe encoded catabolic
pathways. BPA-tolerant strains were isolated from 30% of infant fecal microbial culture libraries
analyzed. Most isolated strains were phylogenetically related to the operational taxonomic group
Bacillus amyloliquefaciens spp. Importantly, WGS analysis of microbial representative strain, Bacillus
sp. AM1 identified the four complete molecular pathways involved on BPA degradation indicating
its versatility and high potential to degrade BPA. Pathways for Exopolysaccharide (EPS) and Polyhydroxyalkanates (PHA) biopolymer synthesis were also identified and phenotypically confirmed by
transmission electronic microscopy (TEM). These microbial biopolymers could generally contribute
to capture and/or deposit xenobiotics.},
organization = {GP/EFSA/ENCO/380 2018/03/G04:
OBEMIRISK: Knowledge platform for assessing the risk of Bisphenols on gut microbiota and its
role in obesogenic phenotype: looking for biomarkers},
organization = {FEDERInfrastructure: IE_2019-198},
organization = {APC was funded by EIN-2019-103082},
publisher = {MDPI},
keywords = {Human microbiota},
keywords = {Bacillus},
keywords = {Bisphenols},
keywords = {Molecular pathways},
keywords = {Enzymes},
keywords = {EPS},
keywords = {PHA},
title = {Representative Bacillus sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation},
doi = {10.3390/ijms22094952},
author = {López Moreno, Ana and Torres Sánchez, Alfonso and Acuña Morales, Inmaculada and Suárez García, Antonio Francisco and Aguilera Gómez, Margarita},
}