@misc{10481/68115,
year = {2021},
month = {3},
url = {http://hdl.handle.net/10481/68115},
abstract = {Considering the high prevalence of cartilage-associated pathologies, low self-repair capacity and limitations of current repair techniques, tissue engineering (TE) strategies have emerged as a promising alternative in this field. Three-dimensional culture techniques have gained attention in recent years, showing their ability to provide the most biomimetic environment for the cells under culture conditions, enabling the cells to fabricate natural, 3D functional microtissues (MTs). In this sense, the aim of this study was to generate, characterize and compare scaffold-free human hyaline and elastic cartilage-derived MTs (HC-MTs and EC-MTs, respectively) under expansion (EM) and chondrogenic media (CM). MTs were generated by using agarose microchips and evaluated ex vivo for 28 days. The MTs generated were subjected to morphometric assessment and cell viability, metabolic activity and histological analyses. Results suggest that the use of CM improves the biomimicry of the MTs obtained in terms of morphology, viability and extracellular matrix (ECM) synthesis with respect to the use of EM. Moreover, the overall results indicate a faster and more sensitive response of the EC-derived cells to the use of CM as compared to HC chondrocytes. Finally, future preclinical in vivo studies are still needed to determine the potential clinical usefulness of these novel advanced therapy products.},
organization = {Instituto de Salud Carlos III

Spanish Government

FIS PI17/0393
PI20/0318},
organization = {Junta de Andalucia

PI-0257-2017
PE-03952019},
organization = {Junta de Andalucia

P18-RT-5059},
organization = {University of Granada 

A-CTS-498-UGR18},
organization = {Junta de Andalucia

A-CTS-498-UGR18},
organization = {FEDER-ERDF funds},
publisher = {MDPI},
keywords = {Microtissues},
keywords = {Tissue engineering},
keywords = {Human hyaline chondrocytes},
keywords = {Human elastic chondrocytes},
keywords = {Extracellular matrix},
keywords = {Organoids},
title = {Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications},
doi = {10.3390/biomedicines9030292},
author = {Sánchez Porras, David and Durand-Herrera, Daniel and Chato Astrain, Jesús and Sánchez-López, José Darío and García García, Óscar Darío and Campos Sánchez, Fernando and Carriel Araya, Víctor},
}