@misc{10481/67928,
year = {2021},
url = {http://hdl.handle.net/10481/67928},
abstract = {Steroid 5-alpha reductases (SRD5As) are responsible for the conversion of testosterone
to dihydrotestosterone, a potent androgen, which is the aetiologic factor of androgenetic alopecia.
This study aimed to compare the SRD5A gene expression suppression activity exerted by Thai rice
bran extracts and their components and investigate the interactional mechanism between bioactive
compounds and SRD5A2 using molecular dynamics (MD) simulation. Bran of Oryza sativa cv. Tubtim
Chumphae (TRB), Yamuechaebia Morchor (YRB), Riceberry (RRB), and Malinil Surin (MRB), all
rice milling by-products, was solvent-extracted. The ethanolic extract of TRB had the highest sum
of overall bioactive compounds (γ-oryzanol; α-, β-, and γ-tocopherol; phenolics; and flavonoids).
Among all extracts, TRB greatly downregulated the expression of SRD5A1, SRD5A2, and SRD5A3;
there were no significant differences between TRB and finasteride regarding SRD5A suppression. The
linear relationship and principal component analysis supported that the α-tocopherol content was
correlated with the SRD5A suppression exerted by TRB. Furthermore, MD simulation demonstrated that α-tocopherol had the highest binding affinity towards SRD5A2 by interacting with residues
Phe118 and Trp201. Our findings indicate that α-tocopherol effectively downregulates the expression
of SRD5A genes and inhibits SRD5A2 activity, actions that are comparable to standard finasteride.
TRB, a source of α-tocopherol, could be developed as an anti-hair loss product.},
organization = {National Research Council of Thailand (NRCT):
NRCT5-RRI63004-P05},
organization = {Chiang Mai University},
publisher = {MDPI},
keywords = {5α-reductase},
keywords = {Androgenetic alopecia},
keywords = {Rice bran},
keywords = {Tocopherol},
keywords = {SRD5A2},
keywords = {Molecular dynamics},
keywords = {RD69},
keywords = {Tubtim chumphae},
title = {Effects on Steroid 5-Alpha Reductase Gene Expression of Thai Rice Bran Extracts and Molecular Dynamics Study on SRD5A2},
doi = {10.3390/biology10040319},
author = {Khantham, Chiranan and Carmona López, Francisco David},
}