@misc{10481/67805,
year = {2021},
month = {2},
url = {http://hdl.handle.net/10481/67805},
abstract = {In critical nerve gap repair, decellularized nerve allografts are considered a promising tissue
engineering strategy that can provide superior regeneration results compared to nerve conduits.
Decellularized nerves offer a well-conserved extracellular matrix component that has proven to
play an important role in supporting axonal guiding and peripheral nerve regeneration. Up to now,
the known decellularized techniques are time and effort consuming. The present study, performed on
rat sciatic nerves, aims at investigating a novel nerve decellularization protocol able to combine an effective
decellularization in short time with a good preservation of the extracellular matrix component.
To do this, a decellularization protocol proven to be efficient for tendons (DN-P1) was compared with
a decellularization protocol specifically developed for nerves (DN-P2). The outcomes of both the
decellularization protocols were assessed by a series of in vitro evaluations, including qualitative
and quantitative histological and immunohistochemical analyses, DNA quantification, SEM and
TEM ultrastructural analyses, mechanical testing, and viability assay. The overall results showed
that DN-P1 could provide promising results if tested in vivo, as the in vitro characterization demonstrated
that DN-P1 conserved a better ultrastructure and ECM components compared to DN-P2.
Most importantly, DN-P1 was shown to be highly biocompatible, supporting a greater number of
viable metabolically active cells.},
organization = {Ministry of Health, Italy},
organization = {Fondazione Cassa di Risparmio di Torino (Turin, Italy) 

2017.AI190.U219
RF: 2016.2388},
organization = {Spanish "Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía y Competitividad (Instituto de Salud Carlos III) 

FIS PI17-0393
FIS PI20-0318},
organization = {Fondo Europeo de Desarrollo Regional ERDF-FEDER European Union},
organization = {Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020), Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía, España 

P18-RT-5059},
organization = {Programa Operativo FEDER Andalucía 2014-2020, Universidad de Granada, Junta de Andalucía, España 

A-CTS-498-UGR18},
organization = {ERDF-FEDER, the European Union},
publisher = {Mdpi},
keywords = {Peripheral nerves},
keywords = {Decellularization},
keywords = {Acellular},
keywords = {Regeneration},
keywords = {Orthopedic trauma},
keywords = {Extracellular-matrix},
title = {Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves},
doi = {10.3390/ijms22052389},
author = {El Soury, Marwa and García García, Óscar Darío and Carriel Araya, Víctor},
}