@misc{10481/67799,
year = {2021},
month = {3},
url = {http://hdl.handle.net/10481/67799},
abstract = {The use of decellularized extracellular matrix (dECM) as a biomaterial has
been an important step forward for the development of functional tissue
constructs. In addition to tissues and organs, cell cultures are gaining a lot of
attention as an alternative source of dECM. In this work, a novel biomimetic
hydrogel is developed based on dECM obtained from mesenchymal stem cells
(mdECM) for cartilage tissue engineering. To this end, cells are seeded under
specific culture conditions to generate an early chondrogenic extracellular
matrix (ECM) providing cues and elements necessary for cartilage
development. The composition is determined by quantitative, histological,
and mass spectrometry techniques. Moreover, the decellularization process is
evaluated by measuring the DNA content and compositional analyses, and
the hydrogel is formulated at different concentrations (3% and 6% w/v).
Results show that mdECM derived hydrogels possess excellent
biocompatibility and suitable physicochemical and mechanical properties for
their injectability. Furthermore, it is evidenced that this hydrogel is able to
induce chondrogenesis of mesenchymal stem cells (MSCs) without
supplemental factors and, furthermore, to form hyaline cartilage-like tissue
after in vivo implantation. These findings demonstrate for the first time the
potential of this hydrogel based on mdECM for applications in cartilage repair
and regeneration.},
organization = {Spanish Ministry of Education, Culture and Sports 

BOE-A-2014-13539},
organization = {Ministerio de Economía, Industria y Competitividad (ERDF funds) 

RTC-2016-5451-1},
organization = {Instituto de Salud Carlos III

FMM-AP17196-2019},
organization = {Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía (ERDF funds) 

B-CTS-230-UGR18
PY18-2470
SOMM17-6109
P18-FR-2465},
organization = {Instituto de Salud Carlos III, ERDF funds 

DTS19/00145},
publisher = {Wiley},
title = {Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem-Cell-Derived ECM for Cartilage Tissue Engineering},
doi = {10.1002/adhm.202001847},
author = {Antich Acedo, Cristina and Jiménez, Gema and Vicente Álvarez-Manzaneda, Juan De and López Ruiz, Elena and Chocarro Wrona, Carlos and Griñán Lisón, Carmen and Carrillo Delgado, Esmeralda Esperanza and Montañez, Elvira and Marchal Corrales, Juan Antonio},
}