@misc{10481/67762,
year = {2020},
month = {1},
url = {http://hdl.handle.net/10481/67762},
abstract = {The encapsulation of cells into biopolymer matrices enables the preparation of engineered substitute tissues.
Here we report the generation of novel 3D magnetic biomaterials by encapsulation of magnetic nanoparticles and
human hyaline chondrocytes within fibrin-agarose hydrogels, with potential use as articular hyaline cartilagelike
tissues. By rheological measurements we observed that, (i) the incorporation of magnetic nanoparticles
resulted in increased values of the storage and loss moduli for the different times of cell culture; and (ii) the
incorporation of human hyaline chondrocytes into nonmagnetic and magnetic fibrin-agarose biomaterials produced
a control of their swelling capacity in comparison with acellular nonmagnetic and magnetic fibrin-agarose
biomaterials. Interestingly, the in vitro viability and proliferation results showed that the inclusion of magnetic
nanoparticles did not affect the cytocompatibility of the biomaterials. What is more, immunohistochemistry
showed that the inclusion of magnetic nanoparticles did not negatively affect the expression of type II collagen of
the human hyaline chondrocytes. Summarizing, our results suggest that the generation of engineered hyaline
cartilage-like tissues by using magnetic fibrin-agarose hydrogels is feasible. The resulting artificial tissues
combine a stronger and stable mechanical response, with promising in vitro cytocompatibility. Further research
would be required to elucidate if for longer culture times additional features typical of the extracellular matrix of
cartilage could be expressed by human hyaline chondrocytes within magnetic fibrin-agarose hydrogels.},
organization = {This study was supported by projects FIS2013-41821-R (Plan
Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía, Industria y Competitividad, MINECO
Spain, cofunded by Fondo Europeo de Desarrollo Regional, FEDER,
European Union), FIS2017-85954-R (Ministerio de Economía, Industria
y Competitividad, MINECO, and Agencia Estatal de Investigación, AEI,
Spain, cofunded by Fondo Europeo de Desarrollo Regional, FEDER,
European Union), and by the Consejería de Salud y Familias, Junta de
Andalucía, Spain, Grant SAS CS PI-0257-2017.},
title = {In vitro characterization of a novel magnetic fibrin-agarose hydrogel for cartilage tissue engineering},
doi = {10.1016/j.jmbbm.2020.103619},
author = {Bonhome Espinosa, Ana Belén and Campos, Fernando and Durand-Herrera, Daniel and Sánchez-López, José Darío and Schaub, Sebastien and García López-Durán, Juan De Dios and López López, Modesto Torcuato and Carriel Araya, Víctor},
}