@misc{10481/67715,
year = {2021},
month = {2},
url = {http://hdl.handle.net/10481/67715},
abstract = {Bioengineered artificial skin substitutes (BASS) are the main treatment used in addition
to autografts when skin injuries involve a large body surface area. Antiseptic/antibiotic treatment
is necessary to prevent infections in the BASS implant area. This study aims to evaluate the effect
of antiseptics and antibiotics on cell viability, structural integrity, and epidermal barrier function
in BASS based on hyaluronic acid during a 28 day follow-up period. Keratinocytes (KTs) and
dermal fibroblasts (DFs) were isolated from skin samples and used to establish BASS. The following
antibiotic/antiseptic treatment was applied every 48 h: colistin (1%), chlorhexidine digluconate
(1%), sodium chloride (0.02%), and polyhexanide (0.1%). Cell viability (LIVE/DEAD® assay),
structural integrity (histological evaluation), and epidermal barrier function (trans-epidermal water
loss, (TEWL), Tewameter®) were also evaluated. Cell viability percentage of BASS treated with
chlorhexidine digluconate was significantly lower (p   0.001) than the other antiseptics at day 28.
Compared to other treatments, chlorhexidine digluconate and polyhexanide significantly affected the
epithelium. No significant differences were found regarding epidermal barrier. These results may be
useful for treatment protocols after implantation of BASS in patients and evaluating them in clinical
practice. BASS represent a suitable model to test in vitro the impact of different treatments of other
skin wounds.},
organization = {Ministry of Science, Innovation and Universities of Spain},
organization = {University of Granada},
organization = {Instituto de Salud Carlos III

PI13/02576
PI17/02083},
organization = {European Regional Development Fund "A way to make Europe"},
organization = {Andalusian Regional Government 

SAS PI-0458-2016},
publisher = {Mdpi},
keywords = {Antiseptic/antibiotic testing},
keywords = {Bioengineered artificial skin substitute},
keywords = {Cell viability},
keywords = {Epidermal barrier function},
keywords = {Regenerative medicine},
keywords = {Wound healing},
title = {Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model},
doi = {10.3390/jcm10040642},
author = {Quiñones Vico, María Isabel and Fernández González, Ana and Pérez Castejón, Elena and Montero Vílchez, Trinidad and Arias Santiago, Salvador Antonio},
}