@misc{10481/67551,
year = {2020},
month = {12},
url = {http://hdl.handle.net/10481/67551},
abstract = {Background: Metformin, a first-line oral antidiabetic agent that has shown promising results in terms of treating
childhood and adolescent obesity, might influence the composition of the gut microbiota. We aimed to evaluate
whether the gut microbiota of non-diabetic children with obesity changes after a metformin intervention.
Methods: The study was a multicenter and double-blind randomized controlled trial in 160 children with obesity.
Children were randomly assigned to receive either metformin (1 g/day) or placebo for 6 months in combination
with healthy lifestyle recommendations in both groups. Then, we conducted a metagenomic analysis in a subsample
obtained from 33 children (15 metformin, 18 placebo). A linear mixed-effects model (LMM) was used to
determine the abundance changes from baseline to six months according to treatment. To analyze the data by
clusters, a principal component analysis was performed to understand whether lifestyle habits have a different
influence on the microbiota depending on the treatment group.
Results: Actinobacteria abundance was higher after placebo treatment compared with metformin. However, the
interaction time x treatment just showed a trend to be significant (4.6% to 8.1% after placebo vs. 3.8 % to 2.6 %
after metformin treatment, p = 0.055). At genus level, only the abundance of Bacillus was significantly higher
after the placebo intervention compared with metformin (2.5% to 5.7% after placebo vs. 1.5 % to 0.8 % after
metformin treatment, p = 0.044). Furthermore, different ensembles formed by Firmicutes, Bacteroidetes, and
Verrucomicrobia were found according to the interventions under a similar food consumption},
organization = {Spanish Ministry of Health, Social and Equality, General Department for Pharmacy and Health Products},
organization = {Instituto de Salud Carlos III-Fondo de Investigación Sanitaria (FONDOS FEDER), Redes temáticas de investigación cooperativa RETIC 

Red SAMID RD12/0026/0015},
publisher = {Elsevier France-Editions Scientifiques Medicales Elsevier},
keywords = {Metformin},
keywords = {Microbiota},
keywords = {Children population},
keywords = {Pubertal stage},
keywords = {Obesity},
title = {Evaluation of the gut microbiota after metformin intervention in children with obesity: A metagenomic study of a randomized controlled trial},
doi = {10.1016/j.biopha.2020.111117},
author = {Pastor Villaescusa, Belén and Plaza Díaz, Julio and Egea Zorrilla, Alejandro and Hoyos, Raúl and Gil Hernández, Ángel and Aguilera García, Concepción María},
}