@misc{10481/67094,
year = {2020},
month = {9},
url = {http://hdl.handle.net/10481/67094},
abstract = {Objective:The  purpose  of this in  vitrostudy  was  to  evaluate  the antibacterial effect  ofanovel non-resorbable,  bioactive polymeric nanostructuredmembrane(NMs), whendoped  with  zinc, calcium and doxycycline.Methods:A validated in vitrosubgingival biofilm model with six bacterial species (Streptococcus oralis, Actinomyces   naeslundii,   Veillonela parvula,   Fusobacterium   nucleatum,   Porphyromonas gingivalis andAggregatibacter actinomycetemcomitans)was used. The experimental NMs, with and  without  beingdoped  with  doxycycline,  calcium  and  zinc,were  placed  on  hydroxyapatite (HA) discs. As positive control membranes, commercially available dense polytetrafluoroethylene (d-PTFE) membranes were used and,as negative controls,the HA discs without any membrane.The experimental, positive and negative control discs were exposed to a mixed bacterial suspension, at 37ºC under anaerobic conditions,during 12, 24, 48 and 72 hours. The resulting biofilms  were analyzed  through scanning  electron  microscopy(SEM),to  study their  structure,andby  quantitative  polymerase  chain  reaction  (qPCR),to  assess the bacterialload,expressed as colony forming units (CFU) per mL. Differences between experimental and control groups were evaluated with the general linear model and the Bonferroni adjustment. Results:As shown by SEM, all membrane groups,except the NMswith doxycycline,resulted in  structured  biofilms from 12  to  72  hours. Similarly,  only  the  membranes  loaded with doxycycline demonstrated a significant reduction in bacterial load during biofilm development,when compared with the control groups (p<0.001).Significance:Doxycycline-doped nanostructured membranes have an impact onbiofilm growth dynamics by significant reducing the bacterial load.},
organization = {This work was supported by the Ministry of Economy and Competitiveness (and European Regional Development Fund [Project MAT2017-85999-PMINECO/AEI/FEDER/UE] and University of Granada Research and Transfer Program.},
title = {[EMBARGADO HAST DIC 2021]Antimicrobial effect of nanostructured membranes for guided tissue regeneration: an in vitro study},
doi = {https://doi.org/10.1016/j.dental.2020.09.011},
author = {Bueno, J. and Sánchez, M. C. and Toledano Osorio, Manuel and Figuero, E and Toledano Pérez, Manuel and Medina Castillo, Antonio Luis and Osorio Ruiz, Raquel and Herrera, D and SAnz, M},
}