@misc{10481/67093,
year = {2020},
month = {9},
url = {http://hdl.handle.net/10481/67093},
abstract = {Objectives: To  evaluate  the  adsorption  of bone related  proteins and bioactivity  ofexperimentalfunctionalized (carboxylated oraminated) polymeric membranes for bone regeneration.Methods: Polymethylmethacrylate-based  membranes functionalized  with  carboxyl  or aminoradicalswere tested. Membranes were zinc loaded and the adsorption isotherms of zinc were studied. A commercially available polytetrafluoroethylene membrane was used  as  control. Human  plasma  proteins, bovine  serum  albumin,  fibrinogen  and fibronectin  adsorption were measured  with  a  spectrophotometer  applying  an  acid determination  protocol.  Biomimetic  calcium  phosphate  precipitation  on  polymeric membraneswas also assessedtroughsimulated   body  fluid   immersion.   Scanning electron  microscopy and  elemental analysis  by means  of  an energy  dispersive  system were used for mineral deposits identification.Results: Both  experimental  membranes  produced  higher  protein  adsorption  than  the commercial  control  that  does  not  adsorb  proteins.   Carboxylated  membranes adsorbed significantly   more   albumin   than   the   aminated   ones, the opposite occurred   with fibrinogen.  With plasma  and  fibronectin  proteins  both  type  of  membranes  performed similarly.Only  carboxylated  membranes  were  bioactive  and  precipitated  calcium  and phosphate  on  their  surfaces. Conclusions: The  polymethylmethacrylate zinc-loaded membranesfunctionalized   with   carboxyl groups   performed   as   highadsorbable membranes  forboneregenerationrelated  proteins.  They also served  as  templates  for mineralization of hydroxyapatite. Clinical significance:Protein adsorption is the initial reaction after the implantation of a biomaterial  into  the  body  and  will  influence  subsequent  cell  function  and  mineral deposition.  Adsorption  of  those  adherent  bone  related  proteins  on  the  implanted material  surface  could  be  tightly  associated  with  its  ability  to  induce  new  bone formation to promote bone regeneration.},
keywords = {Membranes},
keywords = {Bone regeneration},
keywords = {Carboxyl},
keywords = {Amino},
keywords = {Adsorption},
keywords = {Proteins},
title = {Protein adsorption and bioactivity of functionalized electrospun membranes for bone regeneration},
doi = {https://doi.org/10.1016/j.jdent.2020.103473},
author = {Toledano Pérez, Manuel and Carrasco-Carmona, Álvaro and Medina Castillo, Antonio Luis and Toledano Osorio, Manuel and Osorio Ruiz, Raquel},
}