@misc{10481/66989,
year = {2021},
url = {http://hdl.handle.net/10481/66989},
abstract = {In bone tissue engineering, the design of 3D systems capable of recreating composition,
architecture and micromechanical environment of the native extracellular matrix (ECM) is still a
challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical
stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the
culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen
scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues
guiding stem cell fate. The scaffolds were fabricated by mineralization of recombinant collagen in
the presence of magnesium (RCP.MgAp). The organic matrix was homogeneously mineralized with
apatite nanocrystals, similar in composition to those found in bone. X-Ray microtomography images
revealed isotropic porous structure with optimum porosity for cell ingrowth. In fact, an optimal
cell repopulation through the entire scaffolds was obtained after 1 day of dynamic seeding in the
bioreactor. Remarkably, RCP.MgAp scaffolds exhibited higher cell viability and a clear trend of
up-regulation of osteogenic genes than control (non-mineralized) scaffolds. Results demonstrate the
potential of the combination of biomimetic mineralization of recombinant collagen in presence of
magnesium and dynamic culture of hMSC as a promising strategy to closely mimic bone ECM.},
organization = {EU Marie Curie Project "Bio-Inspired Bone Regeneration" 

607051},
organization = {Spanish Ministry of Science, Innovation and Universities (MCIU) 

RYC-2016-21042
RTI-2018-095794A-C22},
organization = {Interdisciplinary Center for Clinical Research (IZKF) at the University ofWuerzburg 

D-361},
organization = {MCIU},
publisher = {MDPI},
keywords = {Scaffold},
keywords = {Perfusion bioreactor},
keywords = {Collagen},
keywords = {Apatite nanoparticles},
keywords = {Magnesium},
keywords = {Human Mesenchymal Stem Cells},
keywords = {Osteogénesis},
title = {Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor},
doi = {10.3390/ijms22031447},
author = {Ramírez Rodríguez, Gloria Belén and Delgado López, José Manuel},
}