@misc{10481/66976,
year = {2021},
month = {1},
url = {http://hdl.handle.net/10481/66976},
abstract = {In this work, calcium oxalate (CaOx) precursors were stabilized by poly(acrylic acid) (PAA)
as an additive under in vitro crystallization assays involving the formation of pre-nucleation clusters
of CaOx via a non-classical crystallization (NCC) pathway. The in vitro crystallization of CaOx was
carried out in the presence of 10, 50 and 100 mg/L PAA by using automatic calcium potentiometric
titration experiments at a constant pH of 6.7 at 20  C. The results confirmed the successful stabilization
of amorphous calcium oxalate II and III (ACOII and ACO III) nanoparticles formed after PNC in
the presence of PAA and suggest the participation and stabilization of polymer-induced liquidprecursor
(PILP) in the presence of PAA. We demonstrated that PAA stabilizes CaOx precursors
with size in the range of 20–400 nm. PAA additive plays a key role in the in vitro crystallization of
CaOx stabilizing multi-ion complexes in the pre-nucleation stage, thereby delaying the nucleation
of ACO nanoparticles. Indeed, PAA additive favors the formation of more hydrated and soluble
phase of ACO nanoparticles that are bound by electrostatic interactions to carboxylic acid groups
of PAA during the post-nucleation stage. These findings may help to a better understanding of the
pathological mineralization resulting in urolithiasis in mammals.},
organization = {Chilean Agency for Research and Development (ANID) - Fondecyt 

1171520},
organization = {ACCDiS center, Fondap 

15130011},
organization = {Comisión Nacional de Investigación Científica y Tecnológica (CONICYT)

21181417},
organization = {Spanish Government

European Commission

RTI2018-099565-B-I00},
organization = {Junta de Andalucía

RNM-179},
organization = {University of Granada (Unidad Científica de Excelencia) 

UCE-PP2016-05},
publisher = {Mdpi},
keywords = {Calcium oxalate},
keywords = {Pre-nucleation clusters},
keywords = {Poly(acrylic acid)},
keywords = {Amorphous calcium oxalate (ACO)},
title = {Stabilization of Calcium Oxalate Precursors during the Pre- and Post-Nucleation Stages with Poly(acrylic acid)},
doi = {10.3390/nano11010235},
author = {Díaz Soler, Felipe and Rodríguez Navarro, Carlos Manuel and Ruiz Agudo, Encarnación and Neira Carrillo, Andrónico},
}