@misc{10481/66862,
year = {2021},
url = {http://hdl.handle.net/10481/66862},
abstract = {Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our
aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and
Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two
concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at
the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences
between SD and RS procedures, but not remarkable changes were induced by GP, which helped to
preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical
properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves
used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived
mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in
all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of
GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight
impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our
novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.},
organization = {Spanish "Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica, Ministerio de Economia y Competitividad (Instituto de Salud Carlos III) 

FIS PI14-1343
FIS PI17-0393
FIS PI20-0318},
organization = {Fondo Europeo de Desarrollo Regional ERDF-FEDER European Union},
organization = {Plan Andaluz de Investigacion, Desarrollo e Innovacion (PAIDI 2020), Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, Junta de Andalucia, Espana 

P18-RT-5059},
organization = {Programa Operativo FEDER Andalucia 2014-2020, Universidad de Granada, Junta de Andalucia, Espana 

A-CTS-498UGR18},
organization = {ERDF-FEDER, the European Union},
publisher = {MDPI},
keywords = {Tissue engineering},
keywords = {Nerve repair},
keywords = {Nerve tissue decellularization},
keywords = {Genipin},
keywords = {Chemical crosslinking},
keywords = {Histology},
keywords = {Natural biomaterials},
keywords = {Biomechanical and structural properties},
keywords = {Cellbiomaterials interactions},
title = {Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves},
doi = {10.3390/ijms22020674},
author = {García García, Óscar Darío and El Soury, Marwa and González Quevedo, David and Sánchez Porras, David and Chato Astrain, Jesús and Campos, Fernando and Carriel Araya, Víctor},
}