@misc{10481/66718,
year = {2020},
month = {12},
url = {http://hdl.handle.net/10481/66718},
abstract = {Transient receptor potential ankyrin member 1 (TRPA1) belongs to the family of thermo TRP cation channels that detect harmful temperatures, acids and numerous chemical pollutants. TRPA1 is expressed in nervous tissue, where it participates in the genesis of nociceptive signals in response to noxious stimuli and mediates mechanical hyperalgesia and allodynia associated with different neuropathies. The glutamate N-methyl-d-aspartate receptor (NMDAR), which plays a relevant role in allodynia to mechanical stimuli, is connected via histidine triad nucleotide-binding protein 1 (HINT1) and type 1 sigma receptor (σ1R) to mu-opioid receptors (MORs), which mediate the most potent pain relief. Notably, neuropathic pain causes a reduction in MOR antinociceptive efficacy, which can be reversed by blocking spinal NMDARs and TRPA1 channels. Thus, we studied whether TRPA1 channels form complexes with MORs and NMDARs that may be implicated in the aforementioned nociceptive signals. Our data suggest that TRPA1 channels functionally associate with MORs, delta opioid receptors and NMDARs in the dorsal root ganglia, the spinal cord and brain areas. These associations were altered in response to pharmacological interventions and the induction of inflammatory and also neuropathic pain. The MOR-TRPA1 and NMDAR-TRPA1 associations do not require HINT1 or σ1R but appear to be mediated by calcium-activated calmodulin. Thus, TRPA1 channels may associate with NMDARs to promote ascending acute and chronic pain signals and to control MOR antinociception.},
organization = {MICINN Plan Nacional I+D+i 	
RT 2018-093677B-100},
organization = {University of Granada 	
PPJIB2019.11},
organization = {MECD 	
FPU 15/02356
FPU16/03213},
publisher = {MDPI},
keywords = {Transient receptor potential A1},
keywords = {Mu opioid receptor},
keywords = {Calmodulin},
keywords = {Glutamate NMDA receptor},
keywords = {Neuropathic pain},
keywords = {Inflammatory pain},
title = {Calmodulin Supports TRPA1 Channel Association with Opioid Receptors and Glutamate NMDA Receptors in the Nervous Tissue},
doi = {10.3390/ijms22010229},
author = {Cortés-Montero, Elsa and Rodríguez-Muñoz, María and Ruiz Cantero, María del Carmen and Cobos del Moral, Enrique José and Sánchez-Blázquez, Pilar and Garzón-Niño, Javier},
}