@misc{10481/66181,
year = {2020},
month = {9},
url = {http://hdl.handle.net/10481/66181},
abstract = {The potential use of microorganisms in the bioremediation of U pollution has been extensively described. However, a lack of knowledge on molecular resistance mechanisms has become a challenge for the use of these technologies. We reported on the transcriptomic and microscopic response of Stenotrophomonas bentonitica BII-R7 exposed to 100 and 250 μM of U. Results showed that exposure to 100 μM displayed up-regulation of 185 and 148 genes during the lag and exponential phases, respectively, whereas 143 and 194 were down-regulated, out of 3786 genes (>1.5-fold change). Exposure to 250 μM of U showed up-regulation of 68 genes and down-regulation of 290 during the lag phase. Genes involved in cell wall and membrane protein synthesis, efflux systems and phosphatases were up-regulated under all conditions tested. Microscopic observations evidenced the formation of U-phosphate minerals at membrane and extracellular levels. Thus, a biphasic process is likely to occur: the increased cell wall would promote the biosorption of U to the cell surface and its precipitation as U-phosphate minerals enhanced by phosphatases. Transport systems would prevent U accumulation in the cytoplasm. These findings contribute to an understanding of how microbes cope with U toxicity, thus allowing for the development of efficient bioremediation strategies.},
organization = {Spanish Ministry (Ministerio de Educacion, Cultura y Deporte) 	
FPU 15/04284
EST 17/00739},
organization = {European Regional Development Fund of the European Commission},
organization = {MIND-661880},
organization = {CGL2014-59616-R},
publisher = {Elsevier},
keywords = {Microbial remediation},
keywords = {RNA-Seq},
keywords = {Biosorption},
keywords = {Biomineralization},
keywords = {Uuranium tolerance},
title = {Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study},
doi = {10.1016/j.jhazmat.2020.123858},
author = {Pinel Cabello, María and Jroundi, Fadwa and Merroun, Mohamed Larbi},
}