@misc{10481/65897,
year = {2020},
month = {11},
url = {http://hdl.handle.net/10481/65897},
abstract = {Zucchini is a vegetable fruit that is very susceptible to postharvest chilling injury, and fruit
ethylene production is correlated with chilling injury sensitivity, such that the more tolerant the
cultivar, the lower is its ethylene production. It is expected that zucchini fruit with reduced sensitivity
to ethylene would have a higher chilling injury tolerance. In this study, we compared the postharvest
fruit quality of wild type and ethylene-insensitive mutant etr2b, in which a mutation was identified in
the coding region of the ethylene receptor gene CpETR2B. Flowers from homozygous WT (wt/wt),
mutant plants in homozygous (etr2b/etr2b) and heterozygous (wt/etr2b) were hand-pollinated, and all
fruits were harvested with the same length, at about 8 days after pollination. After harvesting, fruit of
each genotype was randomly divided in 3 batches of 12 fruits each (four replications with three fruits
each), and then stored at 4  C and 95% RH. At 0, 7, and 14 days after cold storage, each batch was used
to assess ethylene production, respiration rate, weight and firmness loss, chilling injury, and oxidative
stress metabolites. The results showed a lower chilling injury associated with lower cold-induced
ethylene production in the mutant fruit, in comparison with the WT fruit. These data demonstrated
that the ethylene-insensitive etr2b mutant fruit was more tolerant to chilling injury, confirming that
basal ethylene in the still undamaged fruit could function as a modulator of post-harvest chilling
injury. Moreover, the higher chilling tolerance of the etr2b mutant fruit was not associated with MDA
content, but was concomitant with a reduction in the accumulation of hydrogen peroxide in the
refrigerated mutant fruit.},
organization = {European Union (EU)

AGL2014-54598-C2-1-R
AGL2017-82885-C2-1-R
UAL18-BIO-B017B},
organization = {Spanish Government

AGL2014-54598-C2-1-R
AGL2017-82885-C2-1-R
UAL18-BIO-B017B},
organization = {University of Almeria},
publisher = {Mdpi},
keywords = {Cucurbita pepo},
keywords = {Ethylene insensitivity},
keywords = {Ethylene production},
keywords = {Respiration rate},
keywords = {Oxidative stress},
title = {Effect of Ethylene-Insensitive Mutation etr2b on Postharvest Chilling Injury in Zucchini Fruit},
doi = {10.3390/agriculture10110532},
author = {García, Alicia and Garrido Garrido, Dolores},
}