@misc{10481/65577,
year = {2020},
month = {11},
url = {http://hdl.handle.net/10481/65577},
abstract = {In the present study, semi- and interpenetrated polymer network (IPN) systems based on
hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker,
glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and
evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant
di erences between them regarding composition, morphology, and mechanical properties after
physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention
and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN
membranes. The biological performance was evaluated on primary human mesenchymal stem
cells (hMSCs) and the systems revealed no cytotoxic e ect. The excellent biocompatibility of the
systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces.
Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN,
which suggested that these polymeric membranes could be proposed as an e ective promoter system
of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes
can provide a stable and amenable environment for hMSCs support and growth with potential
applications in the biomedical field.},
organization = {La Caixa Foundation

100010434
LCF/BQ/ES16/11570018},
organization = {Spanish Ministry of Economy and Competitiveness 	
RTC-2016-5451-1},
organization = {Instituto de Salud Carlos III

FMM-AP17196-2019},
publisher = {Mdpi},
keywords = {Interpenetrated polymer network},
keywords = {Semi-IPN},
keywords = {Methacrylated hyaluronic acid},
keywords = {Chitosan},
keywords = {Glycerylphytate},
keywords = {Human Mesenchymal Stem Cells},
title = {Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering},
doi = {10.3390/polym12112661},
author = {Mora Boza, Ana and López Ruiz, Elena and Jiménez, Gema and Marchal Corrales, Juan Antonio},
}