@misc{10481/65106,
year = {2020},
month = {5},
url = {http://hdl.handle.net/10481/65106},
abstract = {Significance: Recent generation of bioengineered human skin allowed the efficient treatment
of patients with severe skin defects. However, the optical and biomechanical properties of these
models are not known.
Aim: Three models of bioengineered human skin based on fibrin-agarose biomaterials (acellular,
dermal skin substitutes, and complete dermoepidermal skin substitutes) were generated and
analyzed.
Approach: Optical and biomechanical properties of these artificial human skin substitutes were
investigated using the inverse adding-doubling method and tensile tests, respectively.
Results: The analysis of the optical properties revealed that the model that most resembled the
optical behavior of the native human skin in terms of absorption and scattering properties was the
dermoepidermal human skin substitutes after 7 to 14 days in culture. The time-course evaluation
of the biomechanical parameters showed that the dermoepidermal substitutes displayed significant
higher values than acellular and dermal skin substitutes for all parameters analyzed and did
not differ from the control skin for traction deformation, stress, and strain at fracture break.
Conclusions: We demonstrate the crucial role of the cells from a physical point of view, confirming
that a bioengineered dermoepidermal human skin substitute based on fibrin-agarose biomaterials
is able to fulfill the minimal requirements for skin transplants for future clinical use at
early stages of in vitro development.},
organization = {Ministry of Science, Innovation and Universities of Spain 	
PGC2018-101904-A-I0},
organization = {Instituto de Salud Carlos III (ISCIII), Ministry of Science, Innovation and Universities, through AES 2017 	
AC17/00013},
organization = {Instituto de Salud Carlos III (ISCIII), Ministry of Science, Innovation and Universities within EuroNanoMed framework, EU 	
AC17/00013},
organization = {University of Granada 	
A.TEP.280.UGR18},
organization = {Junta de Andalucía

PE-0395-2019},
organization = {Fundación Benéfica Anticancer San Francisco Javier y Santa Cándida, Granada, Spain},
organization = {OTRI.35A-07},
publisher = {Spie-Soc Photo Optical Instrumentation Engineers},
keywords = {Optical properties},
keywords = {Absorption},
keywords = {Scattering},
keywords = {Bioengineered skin},
keywords = {Fibrin-agarose biomaterial},
title = {Evaluation of the optical and biomechanical properties of bioengineered human skin generated with fibrin-agarose biomaterials},
doi = {10.1117/1.JBO.25.5.055002},
author = {Ionescu, Ana María Andreea and Chato Astrain, Jesús and Cardona Pérez, Juan De La Cruz and Campos, Fernando and Pérez, María M. and Alaminos Mingorance, Miguel and Garzón Bello, Ingrid Johanna},
}