@misc{10481/65031,
year = {2020},
month = {11},
url = {http://hdl.handle.net/10481/65031},
abstract = {Tissue engineering (TE) seeks to fabricate implants that mimic the mechanical
strength, structure, and composition of native tissues. Cartilage TE requires the development
of functional personalized implants with cartilage-like mechanical properties
capable of sustaining high load-bearing environments to integrate into the surrounding
tissue of the cartilage defect. In this study, we evaluated the novel 1,4-butanediol
thermoplastic polyurethane elastomer (b-TPUe) derivative filament as a 3D bioprinting
material with application in cartilage TE. The mechanical behavior of b-TPUe
in terms of friction and elasticity were examined and compared with human articular
cartilage, PCL, and PLA. Moreover, infrapatellar fat pad-derived human mesenchymal
stem cells (MSCs) were bioprinted together with scaffolds. in vitro cytotoxicity, proliferative
potential, cell viability, and chondrogenic differentiation were analyzed by
Alamar blue assay, SEM, confocal microscopy, and RT-qPCR. Moreover, in vivo biocompatibility
and host integration were analyzed. b-TPUe demonstrated a much
closer compression and shear behavior to native cartilage than PCL and PLA, as well as closer tribological properties to cartilage. Moreover, b-TPUe bioprinted scaffolds
were able to maintain proper proliferative potential, cell viability, and supported
MSCs chondrogenesis. Finally, in vivo studies revealed no toxic effects 21 days after
scaffolds implantation, extracellular matrix deposition and integration within the surrounding
tissue. This is the first study that validates the biocompatibility of b-TPUe
for 3D bioprinting. Our findings indicate that this biomaterial can be exploited for the
automated biofabrication of artificial tissues with tailorable mechanical properties
including the great potential for cartilage TE applications.},
organization = {Consejería de Economía, Innovación, Empresas y Universidad de la Junta de Andalucía 	
SOMM17/6109/UGR},
organization = {European Union (EU)

SOMM17/6109/UGR},
organization = {Ministerio de Economía, Industria y Competitividad. Gobierno de España 	
MAT 2016-78778-R
PCIN-2015-051},
organization = {Instituto de Salud Carlos III

FMM-AP17196-2019},
publisher = {Wiley},
keywords = {1,4-butanediol thermoplastic polyurethane},
keywords = {3D bioprinting},
keywords = {Elastomers},
keywords = {MSCs},
keywords = {Tissue enginering},
title = {Validation of the 1,4-butanediol thermoplastic polyurethane as a novel material for 3D bioprinting applications},
doi = {10.1002/btm2.10192},
author = {Chocarro Wrona, Carlos and Vicente Álvarez-Manzaneda, Juan De and Antich Acedo, Cristina and Jiménez González, Gema and Martínez Moreno, Daniel and Carrillo Delgado, Esmeralda Esperanza and Perán, Macarena and López Ruiz, Elena and Marchal Corrales, Juan Antonio},
}