@misc{10481/64667,
year = {2020},
month = {7},
url = {http://hdl.handle.net/10481/64667},
abstract = {Long non-coding RNAs (lncRNAs) are a heterogeneous class of non-coding RNAs whose
biological roles are still poorly understood. LncRNAs serve as gene expression regulators, frequently
interacting with epigenetic factors to shape the outcomes of crucial biological processes, and playing
roles in di erent pathologies including cancer. Over the last years, growing scientific evidence
supports the key role of some lncRNAs in tumor development and proposes them as valuable
biomarkers for the clinic. In this study, we aimed to characterize lncRNAs whose expression is
altered in tumor samples from patients with lung adenocarcinoma (LUAD) compared to adjacent
normal tissue samples. On an RT-qPCR survey of 90 cancer-related lncRNAs, we found one lncRNA,
DLG2-AS1, which was consistently downregulated in 70 LUAD patients. To gain insight into its
biological function, DLG2-AS1 was cloned and successfully re-expressed in LUAD cancer cell lines.
We determined that DLG2-AS1 is not a cis-regulatory element of its overlapping gene DLG2, as their
transcription levels were not correlated, nor did DLG2-AS1 restoration modify the expression of
DLG2 protein. Furthermore, after generating a receiver operating curve (ROC) and calculating the
area under curve (AUC), we found that DLG2-AS1 expression showed high sensitivity and specificity
(AUC = 0.726) for the classification of LUAD and normal samples, determining its value as a potential
lung cancer biomarker.},
organization = {Spanish Ministry of Economy and Business 	
SAF2015-67919-R},
organization = {Junta de Andalucía

CS2016-3
Pl-0245-2017},
organization = {Asociación Española Contra el Cáncer (AECC) Foundation 	
LabAECC2018},
organization = {International Association for the Study of Lung Cancer (IASLC)'s Young Investigator Award 2017},
organization = {Spanish Ministry of Education, Culture and Sports FPU fellowship 	
FPU17/01258
FPU17/00067},
organization = {"Fundacion Benefica Anticancer Santa Candida y San Francisco Javier" predoctoral fellowship},
organization = {Spanish Ministry of Economy and Business FPI fellowship 	
BES-2013-064596},
organization = {La Caixa Foundation

LCF/BQ/DE15/10360019},
organization = {Marie Sklodowska Curie Actions postdoctoral fellowship (H2020-MSCA-IF-2018) 	
837897},
publisher = {Mdpi},
keywords = {Adenocarcinoma of lung},
keywords = {Biomarkers},
keywords = {Tumor},
keywords = {RNA},
keywords = {Long noncoding},
title = {LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma},
doi = {10.3390/cancers12082080},
author = {Arenas Molina, Alberto Manuel and Cuadros Celorrio, Marta Eugenia and Andrades Delgado, Álvaro and García García, Daniel Jesús and F. Coira, Isabel and Rodríguez, María Isabel and Baliñas Gavira, Carlos and Peinado, Paola and Álvarez Pérez, Juan Carlos and P. Medina, Pedro},
}