@misc{10481/64647,
year = {2020},
month = {9},
url = {http://hdl.handle.net/10481/64647},
abstract = {Genome editing technologies not only provide unprecedented opportunities to study
basic cellular system functionality but also improve the outcomes of several clinical
applications. In this review, we analyze various gene editing techniques used to finetune
immune systems from a basic research and clinical perspective. We discuss
recent advances in the development of programmable nucleases, such as zinc-finger
nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered
regularly interspaced short palindromic repeat (CRISPR)-Cas-associated nucleases. We
also discuss the use of programmable nucleases and their derivative reagents such
as base editing tools to engineer immune cells via gene disruption, insertion, and
rewriting of T cells and other immune components, such natural killers (NKs) and
hematopoietic stem and progenitor cells (HSPCs). In addition, with regard to chimeric
antigen receptors (CARs), we describe how different gene editing tools enable healthy
donor cells to be used in CAR T therapy instead of autologous cells without risking
graft-versus-host disease or rejection, leading to reduced adoptive cell therapy costs
and instant treatment availability for patients. We pay particular attention to the delivery
of therapeutic transgenes, such as CARs, to endogenous loci which prevents collateral
damage and increases therapeutic effectiveness. Finally, we review creative innovations,
including immune system repurposing, that facilitate safe and efficient genome surgery
within the framework of clinical cancer immunotherapies.},
organization = {Spanish ISCIII Health Research Fund},
organization = {European Union (EU)

PI12/01097
PI15/02015
PI18/00337
PI18/00330},
organization = {CECEyU and CSyF councils of the Junta de Andalucia FEDER/European Cohesion Fund (FSE) 	
2016000073391-TRA
2016000073332-TRA
PI-57069
PAIDI-Bio326
PI-0014-2016},
organization = {Nicolas Monardes regional Ministry of Health 	
0006/2018},
organization = {Spanish Government

FPU16/05467
FPU17/02268},
organization = {Industrial Doctorate Plan MCI 	
DIN2018-010180},
organization = {SMSI 	
PEJ-2018-001760-A},
organization = {LentiStem Biotech},
publisher = {Frontiers Media SA},
keywords = {Immunotherapy},
keywords = {CARs},
keywords = {Gene editing},
keywords = {Graft-vs-host disease},
keywords = {Base editors},
title = {Using Gene Editing Approaches to Fine-Tune the Immune System},
doi = {10.3389/fimmu.2020.570672},
author = {Pavlovic, Kristina and Tristán Manzano, María and Maldonado Pérez, Noelia and Cortijo Gutierrez, Marina and Sánchez Hernández, Sabina and Justicia Lirio, Pedro and Martín Molina, Francisco and Benabdellah, Karim},
}