@misc{10481/64474,
year = {2020},
month = {7},
url = {http://hdl.handle.net/10481/64474},
abstract = {Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the
supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the
physiological role of SCs are controversial. Here, we study C57BL/6J mice harboring nonfunctional SCAF1, the full
knockout for SCAF1, or the wild-type version of the protein and found that exercise performance is SCAF1 dependent.
By combining quantitative data–independent proteomics, 2D Blue native gel electrophoresis, and functional
analysis of enriched respirasome fractions, we show that SCAF1 confers structural attachment between III2 and IV
within the N-respirasome, increases NADH-dependent respiration, and reduces reactive oxygen species (ROS).
Furthermore, the expression of AOX in cells and mice confirms that CI-CIII superassembly segments the CoQ in
two pools and modulates CI-NADH oxidative capacity},
organization = {MINECO 	
SAF2015-65633-R},
organization = {MCIU 	
RTI2018-099357-B-I00},
organization = {CIBERFES 	
CB16/10/00282},
organization = {Human Frontier Science Program

RGP0016/2018},
organization = {ISCIII-SGEFI/FEDER, ProteoRed 	
ISCIII-IPT13/0001},
organization = {Fundacio MaratoTV3 	
122/C/2015},
organization = {La Caixa Foundation

HR17-00247},
organization = {Ministry of Economy, Industry and Competitiveness (MEIC)},
organization = {Pro-CNIC Foundation},
organization = {MINECO award 	
SEV-2015-0505
 	
MINECO-BIO2015-67580-P
 	
PGC2018-097019-B-I00},
publisher = {Amer Assoc Advancement Science},
title = {Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool},
doi = {10.1126/sciadv.aba7509},
author = {Calvo, Enrique and Cogliati, Sara and Casuso Pérez, Rafael Antonio and Rodríguez Huertas, Jesús Francisco},
}