@misc{10481/64233,
year = {2020},
month = {8},
url = {http://hdl.handle.net/10481/64233},
abstract = {Next generation sequencing methods are widely used in evaluating the structure and functioning of microbial communities, especially those centered on 16S rRNA subunit. Since Illumina Miseq, the most used sequencing platform, does not allow the full sequencing of 16S rRNA gene, this study aims to evaluate whether the choice of different target regions might affect the outcome of microbiome studies regarding soil and saliva samples. V1V3, V3V4, V4V5 and V6V8 domains were studied, finding that while some regions showed differences in the detection of certain bacterial taxa and in the calculation of alpha diversity, especially in soil samples, the overall effect did not compromise the differentiation of any sample type in terms of taxonomic analysis at the genus level. 16S rRNA target regions did affect the detection of specific bacteria related to soil quality and development, and microbial genera used as health biomarkers in saliva. V1V3 region showed the closest similarity to internal sequencing control mock community B, suggesting it might be the most preferable choice regarding data reliability.},
organization = {European Union (EU)
	
3TR-831434},
organization = {Instituto de Salud Carlos III
	
AC18/00008},
organization = {Ministry of Education, Culture and Sport 	
FPU 17/05413
FPU 16/05822},
organization = {University of Almeria},
organization = {Ministry of Economy and Competitiveness of Spain},
organization = {European Union (EU) CGL2015-71709-R SAF2015-71714-R PEJ2018-004702-A},
publisher = {Springer Nature},
keywords = {Microbial community},
keywords = {Metagenomics},
keywords = {Microbiology},
keywords = {Microbiome},
title = {Influence of 16S rRNA target region on the outcome of microbiome studies in soil and saliva samples},
doi = {10.1038/s41598-020-70141-8},
author = {Soriano-Lerma, Ana and Pérez‑Carrasco, Virginia and Sánchez Marañón, Manuel and Ortiz-González, Matilde and Sánchez‑Martín, Victoria and Gijón Martín, Juan José and Navarro-Marí, José María and García-Salcedo, José Antonio and Soriano, Miguel},
}