@misc{10481/64210,
year = {2020},
url = {http://hdl.handle.net/10481/64210},
abstract = {Generation of biocompatible and biomimetic tissue-like biomaterials is crucial to ensure
the success of engineered substitutes in tissue repair. Natural biomaterials able to
mimic the structure and composition of native extracellular matrices typically show
better results than synthetic biomaterials. The aim of this study was to perform an
in vivo time-course biocompatibility analysis of fibrin-agarose tissue-like hydrogels
at the histological, imagenological, hematological, and biochemical levels. Tissue-like
hydrogels were produced by a controlled biofabrication process allowing the generation
of biomechanically and structurally stable hydrogels. The hydrogels were implanted
subcutaneously in 25 male Wistar rats and evaluated after 1, 5, 9, and 12 weeks
of in vivo follow-up. At each period of time, animals were analyzed using magnetic
resonance imaging (MRI), hematological analyses, and histology of the local area in
which the biomaterials were implanted, along with major vital organs (liver, kidney,
spleen, and regional lymph nodes). MRI results showed no local or distal alterations
during the whole study period. Hematology and biochemistry showed some fluctuation
in blood cells values and in some biochemical markers over the time. However, these
parameters were progressively normalized in the framework of the homeostasis process.
Histological, histochemical, and ultrastructural analyses showed that implantation of
fibrin-agarose scaffolds was followed by a progressive process of cell invasion, synthesis
of components of the extracellular matrix (mainly, collagen) and neovascularization.
Implanted biomaterials were successfully biodegraded and biointegrated at 12 weeks
without any associated histopathological alteration in the implanted zone or distal vital
organs. In summary, our in vivo study suggests that fibrin-agarose tissue-like hydrogels
could have potential clinical usefulness in engineering applications in terms of biosafety
and biocompatibility.},
organization = {Spanish Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I+D+i) from the Spanish Ministerio de Ciencia, Innovacion y Universidades (Instituto de Salud Carlos III) (ERDF-FEDER, European Union) 	
FIS PI17/0391
PI17/0393
PI18/332},
organization = {ISCIII thorough AES 2017 	
AC17/00013},
organization = {EuroNanoMed framework 	
AC17/00013},
organization = {Hispanagar, SA, Burgos, Spain, through CDTI, Ministry of Economy and Competitiveness, Spain 	
IDI-20180052},
organization = {Junta de Andalucia

CS PI-0257-2017
PE-0395-2019},
organization = {Ministerio de Economia, Industria y Competitividad, MINECO 	
FIS2017-85954-R},
organization = {Agencia Estatal de Investigacion, AEI, Spain 	
FIS2017-85954-R},
organization = {European Union (EU)

FIS2017-85954-R},
organization = {National Cordoba University, Argentina 	
Secyt 266
HCS 659/2018},
organization = {Programa Operativo Pluriregional de Crecimiento Inteligente (CRIN) 	
IDI-20180052},
organization = {ERDF-FEDER funds, EU 	
IDI-20180052},
publisher = {FRONTIERS MEDIA SA},
keywords = {Fibrin-agarose hydrogels},
keywords = {In vivo biocompatibility},
keywords = {Blood and biochemical profile},
keywords = {Histological assessment},
keywords = {Biodegradation},
keywords = {Tissue enginering},
title = {Evaluation of Fibrin-Agarose Tissue-Like Hydrogels Biocompatibility for Tissue Engineering Applications},
doi = {10.3389/fbioe.2020.00596},
author = {Campos, Fernando and Bonhome Espinosa, Ana Belén and Chato Astrain, Jesús and Sánchez Porras, David and García García, Óscar Darío and Carmona Martos, Ramón and López López, Modesto Torcuato and Alaminos Mingorance, Miguel and Carriel Araya, Víctor and Rodríguez, Ismael Ángel},
}