@misc{10481/64177,
year = {2016},
month = {1},
url = {http://hdl.handle.net/10481/64177},
abstract = {Cancer stem cells are responsible for tumor progression, metastasis, therapy resistance and cancer
recurrence, doing their identification and isolation of special relevance. Here we show that low adherent
breast and colon cancer cells subpopulations have stem-like properties. Our results demonstrate that
trypsin-sensitive (TS) breast and colon cancer cells subpopulations show increased ALDH activity,
higher ability to exclude Hoechst 33342, enlarged proportion of cells with a cancer stem-like cell
phenotype and are enriched in sphere- and colony-forming cells in vitro. Further studies in MDA-MB-231
breast cancer cells reveal that TS subpopulation expresses higher levels of SLUG, SNAIL, VIMENTIN
and N-CADHERIN while show a lack of expression of E-CADHERIN and CLAUDIN, being this profile
characteristic of the epithelial-to-mesenchymal transition (EMT). The TS subpopulation shows CXCL10,
BMI-1 and OCT4 upregulation, differing also in the expression of several miRNAs involved in EMT and/or
cell self-renewal such as miR-34a-5p, miR-34c-5p, miR-21-5p, miR-93-5p and miR-100-5p. Furthermore,
in vivo studies in immunocompromised mice demonstrate that MDA-MB-231 TS cells form more and
bigger xenograft tumors with shorter latency and have higher metastatic potential. In conclusion,
this work presents a new, non-aggressive, easy, inexpensive and reproducible methodology to isolate
prospectively cancer stem-like cells for subsequent biological and preclinical studies.},
organization = {Ministry of Economy and Competitiveness, Instituto de Salud Carlos III (FEDER funds) 	
PI10/02295
PI10/02149},
organization = {Fundacion Progreso y Salud, Consejeria de Salud, Junta de Andalucia (Ministry of Health, Government of Andalucia) 	
PI-0533-2014},
organization = {Consejeria Economia, Innovacion, Ciencia y Empleo, Junta de Andalucia (Ministry of Economy, Innovation, Science and Employment, Government of Andalucia) 	
CTS-6568},
publisher = {Nature Publishing Group},
title = {Low adherent cancer cell subpopulations are enriched in tumorigenic and metastatic epithelial-to-mesenchymal transition-induced cancer stem-like cells},
doi = {10.1038/srep18772},
author = {Morata Tarifa, Cynthia and Jiménez, Gema and García Chaves, María Ángel and Entrena, José M. and Griñán Lisón, Carmen and Aguilera Gómez, Margarita and Picón Ruiz, Manuel and Marchal Corrales, Juan Antonio},
}