@misc{10481/64165,
year = {2020},
month = {1},
url = {http://hdl.handle.net/10481/64165},
abstract = {Here, temperature-sensitive hybrid poly(N-isopropylacrylamide) (pNIPAM) nanosystems with magnetic
response are synthesised and investigated for controlled release of 5-fluorouracil (5FU) and oxaliplatin
(OXA). Initially, magnetic nanoparticles (@Fe3O4) are synthesised by co-precipitation approach and functionalised
with acrylic acid (AA), 3-butenoic acid (3BA) or allylamine (AL) as comonomers. The thermoresponsive
polymer is grown by free radical polymerisation using N-isopropylacrylamide (NIPAM) as
monomer, N,N’-methylenbisacrylamide (BIS) as cross-linker, and 2,2’-azobis(2-methylpropionamidene)
(V50) as initiator. We evaluate particle morphology by transmission electron microscopy (TEM) and particle
size and surface charge by dynamic light scattering (DLS) and Z-potential (ZP) measurements.
These magnetically active pNIPAM@ nanoformulations are loaded with 5-fluorouracil (5FU) and oxaliplatin
(OXA) to determine loading efficiency, drug content and release as well as the cytotoxicity
against T-84 colon cancer cells. Our results show high biocompatibility of pNIPAM nanoformulations
using human blood cells and cultured cells. Interestingly, the pNIPAM@Fe3O4-3BA þ 5FU nanoformulation
significantly reduces the growth of T-84 cells (57% relative inhibition of proliferation). Indeed,
pNIPAM-co-AL@Fe3O4-AA nanosystems produce a slight migration of HCT15 cells in suspension in the
presence of an external magnetic field. Therefore, the obtained hybrid nanoparticles can be applied as
a promising biocompatible nanoplatform for the delivery of 5FU and OXA in the improvement of colon
cancer treatments.},
organization = {MINECO 	
CTQ16-76311},
organization = {Atraccion de Talento fellowship from the Comunidad de Madrid 	
2018-T1/IND-10736},
organization = {Junta de Andalucía

PI-0476-2016
PI-0102-2017},
organization = {Instituto de Salud Carlos III

PI19/01478},
organization = {Ministerio de Educación, Ciencia y Deporte y Competitividad (Spain) 	
FPU16_01716},
publisher = {Taylor y Francis LTD},
keywords = {PNIPAm},
keywords = {Nanosystems},
keywords = {Magnetic nanoparticle},
keywords = {Fluorouracil; oxaliplatin},
keywords = {Colon cancer},
keywords = {External magnetic field},
title = {Magnetically active pNIPAM nanosystems as temperature-sensitive biocompatible structures for controlled drug delivery},
doi = {10.1080/21691401.2020.1773488},
author = {García Pinel, Beatriz and Cabeza, Laura and Ortiz, Raúl and Prados Salazar, José Carlos and Melguizo Alonso, Consolación},
}