@misc{10481/64158,
year = {2020},
url = {http://hdl.handle.net/10481/64158},
abstract = {The oxidative phosphorylation (OXPHOS) system is a dynamic
system in which the respiratory complexes coexist with superassembled quaternary structures called supercomplexes (SCs). The
physiological role of SCs is still disputed. Here, we used zebrafish
to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to
characterize these structures and found similar SCs to those
described in mice, as well as novel SCs including III2 + IV2, I + IV,
and I + III2 + IV2. To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly
factor 1 (scaf1). scaf1 /  fish displayed altered OXPHOS activity
due to the disrupted interaction of complexes III and IV. scaf1 / 
fish were smaller in size and showed abnormal fat deposition and
decreased female fertility. These physiological phenotypes were
rescued by doubling the food supply, which correlated with
improved bioenergetics and alterations in the metabolic gene
expression program. These results reveal that SC assembly by Scaf1
modulates OXPHOS efficiency and allows the optimization of
metabolic resources.},
organization = {Microscopy Imaging Center of the University of Bern},
organization = {Spanish Ministry of Economy and Competitiveness, MINECO 	
SAF2015-65633-R},
organization = {Spanish Ministry of Economy and Competitiveness, MINECO 	
SAF2015-65633-R},
organization = {Human Frontier Science Program

RGP0016/2018},
organization = {European Research Council (ERC)

337703},
organization = {SNF 	
31003A-159721},
organization = {Swiss National Science Foundation (SNSF)

320030_170062},
organization = {MINECO 	
BIO2015-67580-P},
organization = {Carlos III Institute of Health-Fondo de Investigacion Sanitaria) 	
PRB3
IPT17/0019},
organization = {Fundacion La Marato TV3},
organization = {La Caixa Foundation

HR17-00247},
organization = {Ministry of Economy, Industry and Competitiveness (MEIC)},
organization = {Pro-CNIC Foundation},
organization = {Severo Ochoa Center of Excellence (MEIC award) 	
SEV-2015-0505},
publisher = {WILEY},
keywords = {Metabolism},
keywords = {Mitochondria},
keywords = {OXPHOS super-assembly},
keywords = {SCAF1/COX7A2L},
keywords = {Zebrafish},
title = {Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish},
doi = {10.15252/embr.202050287},
author = {García Poyatos, Carolina and Cogliati, Sara},
}