@misc{10481/64045,
year = {2020},
month = {4},
url = {http://hdl.handle.net/10481/64045},
abstract = {Mammals optimize their physiology to the light–dark cycle by
synchronization of the master circadian clock in the brain with
peripheral clocks in the rest of the tissues of the body. Circadian
oscillations rely on a negative feedback loop exerted by the
molecular clock that is composed by transcriptional activators
Bmal1 and Clock, and their negative regulators Period and
Cryptochrome. Components of the molecular clock are expressed
during early development, but onset of robust circadian oscillations
is only detected later during embryogenesis. Here, we have
used na¨ıve pluripotent mouse embryonic stem cells (mESCs) to
study the role of Bmal1 during early development. We found that,
compared to wild-type cells, Bmal12/2 mESCs express higher
levels of Nanog protein and altered expression of pluripotencyassociated
signalling pathways. Importantly, Bmal12/2 mESCs
display deficient multi-lineage cell differentiation capacity during
the formation of teratomas and gastrula-like organoids. Overall, we
reveal that Bmal1 regulates pluripotent cell differentiation and
propose that the molecular clock is an hitherto unrecognized
regulator of mammalian development.},
organization = {Ramon y Cajal grant of the Spanish ministry of economy and competitiveness 	
RYC2012-10019},
organization = {Spanish ministry of economy and competitiveness 	
BFU2016-75233-P},
organization = {Andalusian regional government 	
PC-0246-2017},
organization = {Fundacion Progreso y Salud (FPS)},
organization = {Instituto de Salud Carlos III

European Union (EU)

CPII17/00032
PI17/01574},
organization = {University of Granada},
publisher = {Life Science Alliance LLC},
title = {The molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cells},
doi = {10.26508/lsa.201900535},
author = {Gallardo, Amador and Molina, Aldara and Asenjo, Helena G and Martorell Marugán, Jordi and Montes, Rosa and Ramos Mejía, Verónica and Sánchez Pozo, Antonio and Carmona Sáez, Pedro and López Onieva, Lourdes and Landeira, David},
}