@misc{10481/63407,
year = {2020},
month = {6},
url = {http://hdl.handle.net/10481/63407},
abstract = {Native collagen-based membranes are used to guide bone regeneration; but due to their
rapid biodegradation, this treatment is often unpredictable. The purpose of this study was to
investigate the biodegradability of natural collagen membranes. Three non-cross-linked resorbable
collagen barrier membranes were tested: Derma Fina (porcine dermis), Evolution Standard (equine
pericardium) and Duo-Teck (equine lyophilized collagen felt). 10 × 10 mm2 pieces of membranes
were submitted to three different degradation procedures: (1) hydrolytic degradation in phosphate
buffer solution, (2) enzyme resistance, using a 0.25% porcine trypsin solution, and (3) bacterial
(Clostridium histolyticum) collagenase resistance test. Weight measurements were performed with an
analytic microbalance. Thickness was measured with a digital caliper. Membranes were analyzed at
different time-points, up to 21 d of immersion. A stereomicroscope was used to obtain membranes’
images. ANOVA and Student Newman Keuls were used for mean comparisons (p < 0.05), except
when analyzing differences between time-points within the same membrane and solution where
pair-wise comparisons were applied (p < 0.001). Derma Fina attained the highest resistance to all
degradation challenges. Duo-Teck was the most susceptible membrane to degradation, complete
degradation occurred as soon as 8 h. The bacterial collagenase solution performed as the most
aggressive test as all membranes presented 100% degradation before 21 d.},
organization = {Ministry of Economy and Competitiveness from Spain 	
MINECO/FEDER MAT2017-85999P},
organization = {European Union (EU)

MINECO/FEDER MAT2017-85999P},
organization = {Regional Government of Andalusia Research Fund from Spain 	
A-BIO-157-UGR-18},
publisher = {MDPI},
keywords = {Collagen},
keywords = {Membranes},
keywords = {Biodegradation},
keywords = {Bone regeneration},
title = {Differential Biodegradation Kinetics of Collagen Membranes for Bone Regeneration},
doi = {doi:10.3390/polym12061290},
author = {Toledano Pérez, Manuel and Asady, Samara and Toledano Osorio, Manuel and Benítez García, José A. and Osorio Ruiz, Raquel},
}