@misc{10481/63392,
year = {2020},
month = {7},
url = {http://hdl.handle.net/10481/63392},
abstract = {Verticillium wilt, caused by the pathogen Verticillium dahliae, is extremely devastating to
olive trees (Olea europea). Currently, no successful control measure is available against it. The objective
of this work was to evaluate the antifungal activity of Bacillus velezensis XT1, a well-characterized
salt-tolerant biocontrol strain, against the highly virulent defoliating V. dahliae V024. In vitro, strain
XT1 showed to reduce fungal mycelium from 34 to 100%, depending on if the assay was conducted with
the supernatant, volatile compounds, lipopeptides or whole bacterial culture. In preventive treatments,
when applied directly on young olive trees, it reduced Verticillium incidence rate and percentage
of severity by 54 and ~80%, respectively. It increased polyphenol oxidase (PPO) activity by 395%,
indicating an enhancement of disease resistance in plant tissues, and it decreased by 20.2% the number
of fungal microsclerotia in soil. In adult infected trees, palliative inoculation of strain XT1 in the soil
resulted in a reduction in Verticillium symptom severity by ~63%. Strain XT1 is biosafe, stable in soil
and able to colonize olive roots endophytically. All the traits described above make B. velezensis XT1
a promising alternative to be used in agriculture for the management of Verticillium wilt.},
organization = {Xtrem Biotech S.L},
organization = {European Union SME Instrument 	
XTOnE-774657},
organization = {Spanish Ministry of Economy and Competitiveness 	
SNEO-20161037E},
organization = {Spanish Government

CGL2011-25748},
organization = {Spanish Ministry of Industry, Trade and Tourism (project VertiSOLUTION)},
organization = {Ramon y Cajal program from the Ministry of Economy and Competitiveness 	
RYC-2014-15532},
organization = {CEI-BioTic grant of the BioTic Campus of International Excellence 	
CAEP2-46},
organization = {Ministry of Economy and Competitiveness 	
DI-14-06868},
publisher = {MDPI},
keywords = {Biocontrol},
keywords = {Antifungal activity},
keywords = {Crop protection},
keywords = {Bacillus velezensis},
keywords = {Verticillium dahliae},
keywords = {Olea europaea},
title = {Biological Control of Verticillium Wilt on Olive Trees by the Salt-Tolerant Strain Bacillus velezensis XT1},
doi = {10.3390/microorganisms8071080},
author = {Castro, David and Torres, Marta and Sampedro Quesada, María Inmaculada and Martínez-Checa Barrero, Fernando José and Béjar Luque, María Victoria},
}