@misc{10481/63263,
year = {2020},
month = {6},
url = {http://hdl.handle.net/10481/63263},
abstract = {The capacity of cytotoxic-T lymphocytes to recognize and destroy tumor cells depends
on the surface expression by tumor cells of MHC class I molecules loaded with tumor antigen
peptides. Loss of MHC-I expression is the most frequent mechanism by which tumor cells evade
the immune response. The restoration of MHC-I expression in cancer cells is crucial to enhance
their immune destruction, especially in response to cancer immunotherapy. Using mouse models,
we recovered MHC-I expression in the MHC-I negative tumor cell lines and analyzed their oncological
and immunological profile. Fhit gene transfection induces the restoration of MHC-I expression in
highly oncogenic MHC-I-negative murine tumor cell lines and genes of the IFN-γ transduction signal
pathway are involved. Fhit-transfected tumor cells proved highly immunogenic, being rejected by a
T lymphocyte-mediated immune response. Strikingly, this immune rejection was more frequent in
females than in males. The immune response generated protected hosts against the tumor growth
of non-transfected cells and against other tumor cells in our murine tumor model. Finally, we also
observed a direct correlation between FHIT expression and HLA-I surface expression in human
breast tumors. Recovery of Fhit expression on MHC class I negative tumor cells may be a useful
immunotherapeutic strategy and may even act as an individualized immunotherapeutic vaccine.},
organization = {FEDER funds (EU) from the Instituto de Salud Carlos III 	
PI12/02031
PI14/01978
PI15/00528
PI17/00197
PI19/01179
PT13/0010/0039
PT17/0015/0041},
organization = {Worldwide Cancer Research 	
15-1166},
organization = {Junta de Andalucia

CTS-143
CTS-3952
CVI-4740},
organization = {Instituto de Salud Carlos III},
organization = {Rio-Hortega Contract from ISCIII 	
CM12/00033},
organization = {ibs.Granada Fellowship 	
496},
publisher = {MDPI},
keywords = {MHC-I restoration},
keywords = {Fhit},
keywords = {Antitumor immunity},
keywords = {Immune profile},
keywords = {Cytotoxic T lymphocytes},
keywords = {Immunotherapy},
keywords = {Vaccines},
title = {Restoration of MHC-I on Tumor Cells by Fhit Transfection Promotes Immune Rejection and Acts as an Individualized Immunotherapeutic Vaccine},
doi = {10.3390/cancers12061563},
author = {Garrido Torres-Puchol, Federico and Pulido, María},
}