@misc{10481/63199,
year = {2012},
month = {9},
url = {http://hdl.handle.net/10481/63199},
abstract = {A new class of rhodamines for the application as indicator dyes in fluorescent pH sensors is presented. Their pH-sensitivity derives from photoinduced electron transfer between non-protonated amino groups and the excited chromophore which results in effective fluorescence quenching at increasing pH. The new indicator class carries a pentafluorophenyl group at the 9-position of the xanthene core where other rhodamines bear 2-carboxyphenyl substituents instead. The pentafluorophenyl group is used for covalent coupling to sensor matrices by “click” reaction with mercapto groups. Photophysical properties are similar to “classical” rhodamines carrying 2′-carboxy groups. pH sensors have been prepared with two different matrix materials, silica gel and poly(2-hydroxyethylmethacrylate). Both sensors show high luminescence brightness (absolute fluorescence quantum yield ΦF≈0.6) and high pH-sensitivity at pH 5–7 which makes them suitable for monitoring biotechnological samples. To underline practical applicability, a dually lifetime referenced sensor containing Cr(III)-doped Al2O3 as reference material is presented.},
organization = {Austrian Science Fund (FWF)
	
P 21192-N17},
organization = {Spanish Government
	
AT2009-0019},
publisher = {Elsevier},
keywords = {pH sensor},
keywords = {Fluorescence},
keywords = {Rhodamine},
keywords = {Photoinduced electron transfer},
keywords = {Covalent dye coupling},
keywords = {Click chemistry},
title = {New fluorescent pH sensors based on covalently linkable PET rhodamines},
doi = {10.1016/j.talanta.2012.05.039},
author = {Aigner, Daniel and Borisov, Sergey M. and Orriach Fernández, Francisco José and Fernández Sánchez, Jorge Fernando and Saf, Robert and Klimant, Ingo},
}